PRICKLY PEAR (OPUNTIA SP) PECTIN REVERSES LOW-DENSITY-LIPOPROTEIN RECEPTOR SUPPRESSION INDUCED BY A HYPERCHOLESTEROLEMIC DIET IN GUINEA-PIGS
PRICKLY PEAR (OPUNTIA SP) PECTIN REVERSES LOW-DENSITY-LIPOPROTEIN RECEPTOR SUPPRESSION INDUCED BY A HYPERCHOLESTEROLEMIC DIET IN GUINEA-PIGS
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DOI：
10.1093/jn/122.12.2330
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发表时间：
1992-12-01
影响因子：
4.2
通讯作者：
MCNAMARA, DJ
中科院分区：
文献类型：
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作者：
FERNANDEZ, ML;LIN, ECK;MCNAMARA, DJ
通过给豚鼠饲喂含有 15 g/100 g 猪油和 0.25 g/100 g 胆固醇的饮食（LC 饮食）或用仙人掌果胶部分替代纤维素（2.5 g/100 g）的 LC 饮食（LC-P 饮食），研究仙人掌果胶对血浆 LDL 代谢的影响。 LC-P 饮食使血浆 LDL 胆固醇浓度降低了 33% (P < 0.001)。通过摄入仙人掌果胶改变低密度脂蛋白成分；饲喂 LC-P 饮食的动物的 LDL 中游离胆固醇和酯化胆固醇的相对百分比较低，甘油三酯较高（P < 0.05）。摄入仙人掌果胶不影响肝脏3-羟基-3-甲基戊二酰辅酶A还原酶活性；然而，肝游离胆固醇和酯化胆固醇浓度分别降低了 46% 和 64%。饲喂 LCP 饮食的动物的肝脏载脂蛋白 B/E 受体表达 (B(max)) 高出 60% (P < 0.01)。与体外数据类似，喂食 LC-P 饮食的动物中受体介导的 LDL 分解代谢率高出 190% (P < 0.05)，而载脂蛋白 LDL 通量率不受影响。载脂蛋白 LDL 库大小和分解代谢率分数呈现显着相关性 (r = -0.52，P < 0.01)。这些数据表明，载脂蛋白 B/E 受体表达的增加是主要的代谢反应，摄入仙人掌果胶可降低血浆 LDL 浓度。
The effects of prickly pear pectin on plasma LDL metabolism were investigated by feeding guinea pigs either a diet containing 15 g/100 g lard and 0.25 g/100 g cholesterol (LC diet) or the LC diet in which cellulose was partially replaced (2.5 g/100 g) by prickly pear pectin (LC-P diet). The LC-P diet lowered plasma LDL cholesterol concentrations by 33% (P < 0.001). Low density lipoprotein composition was modified by intake of prickly pear pectin; the relative percentages of free and esterified cholesterol were lower and triglycerides were higher in LDL from animals fed the LC-P diet (P < 0.05). Intake of prickly pear pectin did not affect hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase activity; however, hepatic free and esterified cholesterol concentrations were lowered by 46 and 64%, respectively. Hepatic apolipoprotein B/E receptor expression (B(max)) was 60% higher in animals fed the LCP diet (P < 0.01). Similar to the in vitro data, receptor-mediated LDL fractional catabolic rates were 190% higher in animals fed the LC-P diet (P < 0.05), whereas apolipoprotein LDL flux rates were not affected. Apolipoprotein LDL pool size and fractional catabolic rates exhibited a significant correlation (r = -0.52, P < 0.01). These data indicate that an increase in apolipoprotein B/E receptor expression is a major metabolic response by which intake of prickly pear pectin decreases plasma LDL concentrations.