Role of intraluteal estrogen in the regulation of the rat corpus luteum during pregnancy.
Role of intraluteal estrogen in the regulation of the rat corpus luteum during pregnancy.
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黄体内雌激素在妊娠期大鼠黄体调节中的作用。
DOI：
10.1210/endo-102-4-1176
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
P. Keyes
中科院分区：
文献类型：
--
作者：
G. Gibori;P. Keyes
大鼠黄体的显著芳香化能力和雌二醇维持黄体功能的能力。为了检验这一假设，在妊娠第12天垂体切除术和子宫切除术后，通过Silastic胶囊（20 cm或1 cm长）或双氢睾酮（20 cm胶囊）给予大鼠雌二醇（100 μ g/天）、高或低水平睾酮。垂体切除术和子宫切除术使血清孕酮和雄激素水平、黄体中雌二醇浓度和黄体细胞核中雌二醇受体含量显著降低，并引起黄体停止生长。通过20 cm硅胶胶囊每日给予100 μ g雌二醇或高水平睾酮显著增加了黄体中的雌二醇浓度，从第12天到第15天将血清孕酮维持在与妊娠假手术动物相似的浓度，并增加了黄体中雌二醇受体的核含量。小剂量睾酮胶囊治疗可使血清雄激素和孕酮水平、黄体中雌二醇浓度和黄体生长维持在妊娠假手术动物中观察到的水平。用20厘米双氢睾酮胶囊治疗不能维持孕酮分泌和黄体生长。这些结果表明，雌激素的芳构化形成的黄体内可能发挥重要作用，在调节黄体功能在怀孕期间的大鼠。
The pronounced aromatizing ability of the rat corpus luteum and the ability of estradiol to maintain luteal function. To examine this hypothesis, rats were treated with either estradiol (100 microgram/day), high or low levels of testosterone via Silastic capsules (20 cm or 1 cm in length), or dihydrotestosterone (20-cm capsule) after hypophysectomy and hysterectomy on day 12 of pregnancy. Hypophysectomy and hysterectomy caused serum progesterone and androgen levels, estradiol concentrations in the corpora lutea, and the content of estradiol receptor in luteal cell nuclei to decrease significantly, and caused the cessation of luteal growth. The daily administration of 100 microgram estradiol or of high levels of testosterone via the 20-cm Silastic capsule increased the estradiol concentration in the corpora lutea dramatically, maintained serum progesterone from day 12 through day 15 at concentrations similar to those in pregnant, sham-operated animals, and increased the nuclear content of estradiol receptor in the corpora lutea. Treatment with the small testosterone capsule maintained the serum androgen and progesterone levels, estradiol concentrations in the corpora lutea, and luteal growth at levels observed in pregnant, sham-operated animals. Treatment with the 20-cm dihydrotestosterone capsule did not sustain progesterone secretion and luteal growth. These results suggest that estrogen formed by aromatization within the corpora lutea may play an important role in the regulation of luteal function during pregnancy in the rat.