Biochemical changes in progressive muscular dystrophy. XV. Distribution of radioactive glutamate and proximate composition of various components of skeletal muscle and liver in vitamin E-deficient dystrophic rabbits and 129/ReJ (dy/dy) mice.
Biochemical changes in progressive muscular dystrophy. XV. Distribution of radioactive glutamate and proximate composition of various components of skeletal muscle and liver in vitamin E-deficient dystrophic rabbits and 129/ReJ (dy/dy) mice.
复制标题
进行性肌营养不良症的生化变化。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
T. Goswami
中科院分区：
文献类型：
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作者：
U. Srivastava;T. Goswami
在维生素E缺乏的兔子和遗传性营养不良（dy/dy）小鼠在疾病的各个阶段的骨骼肌和肝脏的近似组成和放射性谷氨酸的掺入到它们的各种成分进行了研究。骨骼肌中的总脂肪含量在两种类型的营养不良的早期阶段减少，并在终末期增加。该成分仅在营养不良小鼠的肝脏中发生变化，在轻度阶段显示减少，但稍后恢复到正常值。在疾病的每个阶段，这两个物种的骨骼肌中的碳水化合物含量减少。然而，在肝脏中，它在维生素E缺乏的兔子中显著升高，但在营养不良的小鼠中被抑制。两种营养不良模型的骨骼肌蛋白质含量均降低。氨基酸也得到了类似的结果，只是它们在营养不良的终末阶段增加。在营养诱导和遗传性营养不良（分别在兔和小鼠中）中记录了肝脏氨基酸的类似值。在这两种模型中，将C14-谷氨酸盐掺入骨骼肌和肝脏的各种组分中显示了不同的反应，这取决于营养不良的性质，以及测量掺入的组分。这些数据表明，维生素E缺乏和遗传性营养不良动物的骨骼肌和肝脏中C14-谷氨酸盐的快速周转。此外，很明显，骨骼肌和肝脏的各种成分的变化，以及C14-谷氨酸掺入的模式，在两种类型的营养不良中是不同的。
The proximate composition of skeletal muscle and liver and the incorporation of radioactive glutamate into their various components were investigated in vitamin E-deficient rabbits and genetically dystrophic (dy/dy) mice during various stages of the disease. Total fat content in skeletal muscle was decreased in the early phase and increased in the terminal stage of both types of dystrophy. This component altered only in the liver of dystrophic mice, showing a reduction in the mild stage but returning later to normal values. Carbohydrate content was diminished in the skeletal muscle of both species at each stage of the disease. In the liver, however, it was elevated considerably in vitamin E-deficient rabbits, but was depressed in dystrophic mice. Protein content was decreased in the skeletal muscle of both dystrophic models. Similar results were obtained for amino acids, except that they were increased in the terminal stages of nutritional dystrophy. Analogous values were recorded for hepatic amino acids in nutritionally-induced and hereditary dystrophy (in rabbits and mice respectively). Incorporation of C14-glutamate into various components of skeletal muscle and liver in both models revealed divergent responses, depending upon the nature of the dystrophy, and the component in which incorporation was measured. These data indicate a rapid turnover of C14-glutamate in the skeletal muscle and liver of vitamin E-deficient and genetically dystrophic animals. Also, it is clear that the changes in the various components of skeletal muscle and liver, as well as the pattern of C14-glutamate incorporation, are different in the two types of dystrophy.