BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction
BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction
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DOI：
10.1152/ajprenal.00222.2006
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发表时间：
2007-02-01
影响因子：
4.2
通讯作者：
Becker, Bryan N.
中科院分区：
文献类型：
--
作者：
Djamali, Arjang;Sadowski, Elizabeth A.;Becker, Bryan N.
血氧水平依赖（BOLD）磁共振成像（MRI）使用脱氧血红蛋白作为内源性造影剂，用于无创评估组织氧的生物利用度。我们假设慢性同种异体肾病（CAN）患者的肾内氧合受损。10名患有CAN的肾移植受者和9名健康志愿者进行了BOLD-MRI检查。髓质R2* (MR2*)和皮质R2* (CR2*)水平（与组织脱氧血红蛋白水平成正比）与尿液和血清氧化应激（OS）标志物：过氧化氢（H2O2）、f2 -异前列腺素、总一氧化氮（NO）、热休克蛋白27 （HSP27）和总抗氧化性能（TAOP）一起测定。与健康志愿者相比，CAN患者MR2*和CR2*平均水平显著降低（局部氧合血红蛋白浓度升高）（20.7 +/- 1.6 vs. 23.1 +/- 1.8/ s, P = 0.03; 15.9 +/- 1.9 vs. 13.6 +/- 2.3/ s, P = 0.05）。CAN患者血清和尿液中H2O2水平和血清HSP27水平显著升高。相反，健康志愿者的尿NO水平和TAOP显著升高。多元线性回归分析显示MR2*和CR2*水平与OS的血清/尿液生物标志物有显著相关性。BOLD- MRI显示同种异体CAN移植物髓质和皮质氧生物利用度有显著变化。这些与OS的血清/尿液生物标志物相关，提示肾内氧合与OS之间存在关联。
Blood oxygen level-dependent ( BOLD) magnetic resonance imaging (MRI) uses deoxyhemoglobin as an endogenous contrast agent for the noninvasive assessment of tissue oxygen bioavailability. We hypothesized that intrarenal oxygenation was impaired in patients with chronic allograft nephropathy ( CAN). Ten kidney-transplant recipients with CAN and nine healthy volunteers underwent BOLD-MRI. Medullary R2* ( MR2*) and cortical R2* (CR2*) levels ( measures directly proportional to tissue deoxyhemoglobin levels) were determined alongside urine and serum markers of oxidative stress ( OS): hydrogen peroxide (H2O2'), F2-isoprostanes, total nitric oxide ( NO), heat shock protein 27 (HSP27), and total antioxidant property (TAOP). Mean MR2* and CR2* levels were significantly decreased in CAN ( increased local oxyhemoglobin concentration) compared with healthy volunteers (20.7 +/- 1.6 vs. 23.1 +/- 1.8/ s, P = 0.03 and 15.9 +/- 1.9 vs. 13.6 +/- 2.3/ s, P = 0.05, respectively). There was a significant increase in serum and urine levels of H2O2 and serum HSP27 levels in patients with CAN. Conversely, urine NO levels and TAOP were significantly increased in healthy volunteers. Multiple linear regression analyses showed a significant association between MR2* and CR2* levels and serum/ urine biomarkers of OS. BOLD- MRI demonstrated significant changes in medullary and cortical oxygen bioavailability in allografts with CAN. These correlated with serum/ urine biomarkers of OS, suggesting an association between intrarenal oxygenation and OS.