The effect of selected Lactobacillus strains on dextran sulfate sodium-induced mouse colitis model
The effect of selected Lactobacillus strains on dextran sulfate sodium-induced mouse colitis model
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DOI：
10.1556/030.2020.00834
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发表时间：
2020-06-01
影响因子：
1.5
通讯作者：
Talebi, Malihe
中科院分区：
文献类型：
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作者：
Hasannejad-Bibalan, Meysam;Mojtahedi, Ali;Talebi, Malihe
炎症性肠病（IBD）包括两种主要疾病：克罗恩病（CD）和溃疡性结肠炎（UC）。葡聚糖硫酸钠（DSS）小鼠结肠炎模型已被用于了解IBD的机制。进行本研究以检查选定的乳杆菌属。作为DSS诱导的动物模型中的潜在IBD治疗。使用Balb/c小鼠，通过向饮用水中添加5%葡聚糖硫酸钠8天来诱导结肠炎。测量结肠长度、疾病活动指数（DAI）和组织学分析作为DSS结肠炎小鼠中的炎症标志物。与DSS组相比，大多数乳杆菌属物种显著防止结肠长度缩短。在使用四种乳杆菌菌株（包括植物乳杆菌03和06、短乳杆菌02和鼠李糖乳杆菌01）后，小鼠的DAI评分显著降低。组织学分析表明，口服乳酸杆菌菌株对小鼠结肠炎具有治疗作用。L.植物L.对DSS诱导的小鼠急性结肠炎有较好的治疗作用。这三种分离物的益生菌活性表明益生菌效应是菌株特异性的，并且这些有用的细菌中没有一种可以同时表现出所有有价值的益生菌特性。
Inflammatory bowel disease (IBD) comprises two major illnesses: Crohn's disease (CD) and ulcerative colitis (UC). Dextran sulfate sodium (DSS) mouse colitis model has been used in understanding the mechanism of IBD. This study was conducted to examine selected Lactobacillus spp. as potential IBD treatment in the DSS-induced animal model. Balb/c mice were used and colitis was induced by adding 5% dextran sodium sulfate into the drinking water for 8 days. Colon length, disease activity index (DAI) and histological analysis were measured as markers of inflammation in DSS colitis mice. The majority of the Lactobacillus species significantly prevented the shortening of the colon length compared with the DSS group. The DAI scores of mice were significantly reduced following usage of four Lactobacillus strains included: Lactobacillus plantarum 03 and 06, Lactobacillus brevis 02 and Lactobacillus rhamnosus 01. The histological analysis exhibited that oral administration of Lactobacillus strains had therapeutic effects on mice colitis. L. plantarum and L. brevis showed better therapeutic effect against DSS-induced acute colitis mice. The probiotic activities of these three isolates indicated that the probiotic effects were strain specific and none of these useful bacteria could exhibit all of the valued probiotic properties simultaneously.