Raman tweezers spectroscopy of live, single red and white blood cells.
Raman tweezers spectroscopy of live, single red and white blood cells.
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DOI：
10.1371/journal.pone.0010427
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发表时间：
2010-04-29
期刊：
影响因子：
3.7
通讯作者：
Mathur D
中科院分区：
文献类型：
--
作者：
Bankapur A;Zachariah E;Chidangil S;Valiathan M;Mathur D
将光学陷阱与拉曼光谱仪相结合，高分辨率测量了生理条件下光固定、单一、活红(RBC)和白细胞(WBC)的拉曼光谱。紧聚焦的近红外波长光(1064 Nm)被用来捕获单细胞，785 nm光被用来在低入射功率(几mW)下进行拉曼激发。使用这种高灵敏度的双波长仪器记录的红细胞的拉曼光谱已经能够识别出几条额外的谱线；到目前为止尚未报道的谱线纯粹来自于血红蛋白分子。根据标准的蛋白质和核酸振动光谱数据对单个粒细胞和淋巴细胞的拉曼光谱进行了解释。测量光谱的丰富性表明，悬浮液中活细胞的拉曼研究比传统的显微拉曼研究更具信息量，传统的显微拉曼研究将细胞化学结合在玻璃片上。
An optical trap has been combined with a Raman spectrometer to make high-resolution measurements of Raman spectra of optically-immobilized, single, live red (RBC) and white blood cells (WBC) under physiological conditions. Tightly-focused, near infrared wavelength light (1064 nm) is utilized for trapping of single cells and 785 nm light is used for Raman excitation at low levels of incident power (few mW). Raman spectra of RBC recorded using this high-sensitivity, dual-wavelength apparatus has enabled identification of several additional lines; the hitherto-unreported lines originate purely from hemoglobin molecules. Raman spectra of single granulocytes and lymphocytes are interpreted on the basis of standard protein and nucleic acid vibrational spectroscopy data. The richness of the measured spectrum illustrates that Raman studies of live cells in suspension are more informative than conventional micro-Raman studies where the cells are chemically bound to a glass cover slip.