LIGAND-INDEPENDENT ACTIVATION OF THE SEVENLESS RECEPTOR TYROSINE KINASE CHANGES THE FATE OF CELLS IN THE DEVELOPING DROSOPHILA EYE
LIGAND-INDEPENDENT ACTIVATION OF THE SEVENLESS RECEPTOR TYROSINE KINASE CHANGES THE FATE OF CELLS IN THE DEVELOPING DROSOPHILA EYE
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DOI：
10.1016/0092-8674(91)90262-w
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发表时间：
1991-03-22
期刊：
影响因子：
64.5
通讯作者：
HAFEN, E
中科院分区：
文献类型：
--
作者：
BASLER, K;CHRISTEN, B;HAFEN, E
发育中的眼睛中的细胞命运是由一连串的诱导相互作用决定的。在这个过程中，7 - less蛋白——一种受体酪氨酸激酶——是R7感光细胞命运规范所必需的。我们通过在通常表达sevenless的细胞中过表达截断的sevenless蛋白，构建了一个功能获得的seven - less突变（Sev(S11)）。在Sev（S11）突变果蝇中，所有7个表达较少的细胞启动神经发育。这导致每个小眼形成多个r7样光感受器。因此，seven - less活性似乎是决定R7细胞命运的必要和充分条件。这些结果说明了受体酪氨酸激酶在发育过程中对细胞命运的规范中所起的中心作用。
Cell fate in the developing eye is determined by a cascade of inductive interactions. In this process, the sevenless protein-a receptor tyrosine kinase-is required for the specification of the R7 photoreceptor cell fate. We have constructed a gain-of-function sevenless mutation (Sev(S11)) by overexpressing a truncated sevenless protein in the cells where sevenless is normally expressed. In Sev(S11) mutant flies, all sevenless-expressing cells initiate neural development. This results in the formation of multiple R7-like photoreceptors per ommatidium. Therefore, sevenless activity appears to be necessary and sufficient for the determination of R7 cell fate. These results illustrate the central role receptor tyrosine kinases can play in the specification of cell fate during development.