HIGHLY ELECTRICALLY INSULATING TETHERED LIPID BILAYERS FOR PROBING THE FUNCTION OF ION CHANNEL PROTEINS
HIGHLY ELECTRICALLY INSULATING TETHERED LIPID BILAYERS FOR PROBING THE FUNCTION OF ION CHANNEL PROTEINS
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DOI：
10.1021/la034197v
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发表时间：
2003-06
期刊：
影响因子：
3.9
通讯作者：
S. Terrettaz;Michael Mayer;H. Vogel
中科院分区：
文献类型：
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作者：
S. Terrettaz;Michael Mayer;H. Vogel
提出了一种形成具有极高电阻的金电极束缚的脂质双层的方法。电阻抗谱用于监测配体门控离子通道蛋白的双层掺入和通过抗体的选择性结合对其通道活性的调节。由于系留膜的缺陷密度低，可以解决少数通道的影响，从而为在这个高度稳定和多功能的平台上进行单通道实验开辟了道路。反过来，可以测量微量的分析物，这里是抗体，这对生物分析非常重要。
A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high electrical resistances. Electrical impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channel activity by the selective binding of an antibody. Due to the low defect density of the tethered membrane, the effect of a few channels can be resolved thus opening the way to single-channel experiments on this highly stable and versatile platform. In turn, a minute quantity of analyte, here antibodies, can be measured, which is of great interest for bioanalytics.