PATL2 regulated the apoptosis of ovarian granulosa cells in patients with PCOS
PATL2 regulated the apoptosis of ovarian granulosa cells in patients with PCOS
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DOI：
10.1080/09513590.2021.1928066
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发表时间：
2021-05-11
影响因子：
2
通讯作者：
Shu, Kuan-Yong
中科院分区：
文献类型：
--
作者：
Peng, Shao-Lan;Wu, Qiong-Fang;Shu, Kuan-Yong
目的多囊卵巢综合征（PCOS）常表现为卵泡发育异常。已有研究发现，颗粒细胞凋亡增加是导致卵泡发育不良的关键因素之一。研究发现，PATL2功能的降低或缺失可显著抑制人卵母细胞的发育和成熟。我们发现PATL2在人卵巢GCs中也有表达，提示PATL2可能参与了GCs相关生物学事件的调控。本研究旨在探讨PATL2在GCs凋亡调控中的作用，以及PATL2在PCOS相关异常卵泡发育中的作用。材料与方法收集PCOS患者和正常排卵妇女的卵泡GCs。此外，人颗粒细胞系（KGN）用于体外实验。结果（1）PCOS组卵母细胞成熟率和受精率明显低于正常对照组（p < 0.05）。(2)流式细胞术和TUNEL染色显示PCOS组胃癌细胞凋亡水平明显增高。(3)免疫荧光和Western Blot显示PCOS组GCs的PATL2表达水平明显降低。(4)siRNA抑制PATL2表达可显著抑制GC凋亡。结论PATL2表达降低可导致卵巢GCs凋亡水平升高，这可能与PCOS患者异常卵泡的发生、发展密切相关。
Aim PCOS often showed abnormal follicular development. Previous studies have found that the increased apoptosis of granulosa cells (GCs) is one of the key factors leading to follicular dysplasia. It has been found that the decrease or deletion of PATL2 function can significantly inhibit the development and maturation of human oocytes. We found that PATL2 was also expressed in human ovarian GCs, suggesting that PATL2 may be involved in the regulation of related biological events in GCs. This study aims to explore the function of PATL2 on regulation of GCs apoptosis, and the potential role of PATL2 in the development of PCOS-related abnormal follicles. Materials and Methods The follicular GCs of PCOS patients and normal ovulating female patients were collected. Moreover, human granular cell line (KGN) was used for in vitro experiments. Results (1) The maturation rate and fertilization rate of oocytes in the PCOS group were significantly lower than those in the normal control group (p < 0.05). (2) Flow cytometry and TUNEL staining showed that the apoptosis level of GCs in the PCOS group was significantly increased. (3) Immunofluorescence and Western Blot showed that the PATL2 expression level of GCs in the PCOS group was significantly reduced. (4) Knocking down the expression of PATL2 by siRNA significantly prevented the apoptosis of GCs. Conclusions Reduced PATL2 could resulted in the increased apoptosis level of ovarian GCs, which might be closely related to the occurrence and development of abnormal follicles in PCOS.