Expression mapping of 5-HT1 serotonin receptor subtypes during fetal and early postnatal mouse forebrain development
Expression mapping of 5-HT1 serotonin receptor subtypes during fetal and early postnatal mouse forebrain development
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DOI：
10.1016/j.neuroscience.2006.04.036
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Levitt, P.
中科院分区：
文献类型：
--
作者：
Bonnin, A.;Peng, W.;Levitt, P.
5-羟色胺（5-HT）与脑发育的几个方面有关，但负责转导特异性效应的5-HT受体的个体发育表达模式在很大程度上还没有被表征。15个不同的5-HT受体基因已被克隆，因此任何空间和/或时间的组合，其发育表达可以介导广泛的5-HT的影响。我们进行了详细的分析，在胎儿和出生后早期小鼠前脑的Gi/ocoupled 5-HT 1（5-HT 1A，1B，1D和1F）受体亚型的表达图谱。使用受体亚型特异性核糖探针和原位杂交，我们观察到所有5-HT 1受体亚型早在胚胎第14.5天（E）就在前脑中表达，通常在特定结构内呈梯度表达。在5-HT 1受体中，5-HT 1A受体转录物在E14.5-16.5丘脑、海马和皮层中以内侧到外侧的梯度密集表达，而5-HT 1B受体mRNA在这些年龄的背侧丘脑的更外侧部分和纹状体中表达。5-HT 1D受体转录本也在E14.5-E16.5丘脑中大量表达，似乎在出生时下调。5-HTIF受体转录本存在于E14.5-16.5时的增殖区域，如皮质脑室区、神经节隆起和丘脑内侧，并且在其他方面与5-HTIB和1D受体转录本的表达模式相似。总体而言，5-HT 1亚家族的Gi/o-偶联5-HT受体显示特定的和动态的表达模式在胚胎前脑发育。此外，5-HT 1受体类的所有成员都在胚胎背侧丘脑中强烈和短暂表达，这表明5-羟色胺在早期丘脑发育中的潜在作用。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Serotonin (5-HT) is implicated in several aspects of brain development, yet the ontogenetic expression patterns of 5-HT receptors responsible for transducing specific effects have largely not been characterized. Fifteen different 5-HT receptor genes have been cloned; therefore any spatial and/or temporal combination of their developmental expression could mediate a wide array of 5-HT effects. We undertook a detailed analysis of expression mapping of the Gi/ocoupled 5-HT1 (5-HT1A, 1B, 1D and 1F) receptor subtypes in the fetal and early postnatal mouse forebrain. Using receptor subtype-specific riboprobes and in situ hybridization, we observed that all 5-HT1 receptor subtypes are expressed as early as embryonic day (E) 14.5 in the forebrain, typically in gradients within specific structures. Among 5-HT1 receptors, the 5-HT1A receptor transcript is expressed densely in E14.5-16.5 thalamus, in hippocampus, and in a medial to lateral gradient in cortex, whereas the 5-HT1B receptor mRNA is expressed in more lateral parts of the dorsal thalamus and in the striaturn at these ages. The 5-HT1D receptor transcript, which also is expressed heavily in E14.5-E16.5 thalamus, appears to be down-regulated at birth. The 5-HTIF receptor transcript is present in proliferative regions such as the cortical ventricular zone, ganglionic eminences, and medial aspects of the thalamus at E14.5-16.5, and otherwise presents similarities to the expression patterns of 5-HTIB and 1D receptor transcripts. Overall, the 5-HT1 subfamily of Gi/o-coupled 5-HT receptors displays specific and dynamic expression patterns during embryonic forebrain development. Moreover, all members of the 5-HT1 receptor class are strongly and transiently expressed in the embryonic dorsal thalamus, which suggests a potential role for serotonin in early thalamic development. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.