Proteomic analysis of Sulfolobus solfataricus during Sulfolobus Turreted Icosahedral Virus infection.
Proteomic analysis of Sulfolobus solfataricus during Sulfolobus Turreted Icosahedral Virus infection.
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DOI：
10.1021/pr201087v
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发表时间：
2012-02
影响因子：
4.4
通讯作者：
Walid Maaty;Kyla Selvig;S. Ryder;P. Tarlykov;J. Hilmer;Joshua Heinemann;J. Steffens;Jamie C. Snyder;A. C. Ortmann;Navid Movahed;K. Spicka;L. Chetia;P. Grieco;E. Dratz;T. Douglas;M. Young;B. Bothner
中科院分区：
文献类型：
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作者：
Walid Maaty;Kyla Selvig;S. Ryder;P. Tarlykov;J. Hilmer;Joshua Heinemann;J. Steffens;Jamie C. Snyder;A. C. Ortmann;Navid Movahed;K. Spicka;L. Chetia;P. Grieco;E. Dratz;T. Douglas;M. Young;B. Bothner
有生命的地方就有病毒。病毒对进化、全球营养循环和疾病的影响推动了对其细胞和分子生物学的研究。我们对各种病毒都有了解；然而，一个主要的例外是带有古细菌宿主的病毒。古细菌病毒-宿主系统非常有趣，因为它们与真核生物和细菌系统有相似之处，并且经常生活在极端环境中。在这里，我们报告了第一个基于蛋白质组学的古细菌宿主对病毒感染反应的实验。采用1D和2D差分凝胶电泳（DIGE）技术检测了硫虫转塔二十面体病毒（STIV）侵染硫虫P2的丰度和氧化还原变化。使用新型荧光两性离子化学探针测量了半胱氨酸反应性，该探针与丰度变化一起表明，病毒和宿主都在争夺对细胞氧化还原状态的控制。来自近50%的预测病毒开放阅读框的蛋白质与STIV v2中具有同源物的新STIV蛋白一起被发现。这项研究提供了对古细菌病毒复制的新特征的见解，与真核病毒（如ESCRT-III介导的转运）使用的良好描述机制有很强的联系，并强调了不同组学方法的互补性。
Where there is life, there are viruses. The impact of viruses on evolution, global nutrient cycling, and disease has driven research on their cellular and molecular biology. Knowledge exists for a wide range of viruses; however, a major exception are viruses with archaeal hosts. Archaeal virus-host systems are of great interest because they have similarities to both eukaryotic and bacterial systems and often live in extreme environments. Here we report the first proteomics-based experiments on archaeal host response to viral infection. Sulfolobus Turreted Icosahedral Virus (STIV) infection of Sulfolobus solfataricus P2 was studied using 1D and 2D differential gel electrophoresis (DIGE) to measure abundance and redox changes. Cysteine reactivity was measured using novel fluorescent zwitterionic chemical probes that, together with abundance changes, suggest that virus and host are both vying for control of redox status in the cells. Proteins from nearly 50% of the predicted viral open reading frames were found along with a new STIV protein with a homologue in STIV2. This study provides insight to features of viral replication novel to the archaea, makes strong connections to well-described mechanisms used by eukaryotic viruses such as ESCRT-III mediated transport, and emphasizes the complementary nature of different omics approaches.