PHOTOSTABILITY STUDIES OF PHYCOBILIPROTEIN FLUORESCENT LABELS
PHOTOSTABILITY STUDIES OF PHYCOBILIPROTEIN FLUORESCENT LABELS
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DOI：
10.1016/0003-2697(87)90473-8
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发表时间：
1987-03-01
影响因子：
2.9
通讯作者：
STRYER, L
中科院分区：
文献类型：
--
作者：
WHITE, JC;STRYER, L
对四种荧光藻胆蛋白进行了光稳定性研究，以确定生物标记应用的稳定发色团。藻胆蛋白的光破坏与所施加的激光功率呈线性关系，并依赖于每个分子吸收的光子总数。光破坏量子产率的1.1倍。10-5对于R-藻红蛋白，6.6 ×10-6对于B-藻红蛋白，4.5 ×10-6对于别藻蓝蛋白，和2.5 ×10-5测定了C-藻蓝蛋白。C-藻蓝蛋白的光稳定性是荧光素的10.8倍。没食子酸正丙酯的加入使藻红蛋白的光稳定性提高了1.7倍。加入超氧化物歧化酶、过氧化氢酶、连二亚硫酸钠、抗坏血酸、二硫苏糖醇、EDTA或氩气鼓泡脱氧对R-藻红蛋白的光稳定性没有影响。
Photostability studies of four fluorescent phycobiliproteins were conducted to identify stable chromophores for biological labeling applications. Phycobiliprotein photodestruction was linear with the applied laser power and depended on the total number of photons absorbed per molecule. Photodestruction quantum yields .vphi. of 1.1 .times. 10-5 for R-phycoerythrin, 6.6 .times. 10-6 for B-phycoerythrin, 4.5 .times. 10-6 for allophycocyanin, and 2.5 .times. 10-5 for C-phycocyanin were measured. C-Phycocyanin is a factor of 10.8 more photostable than fluorescein. The photostability of R-phycoerythrin was improved by a factor of 1.7 by adding n-propyl gallate. The addition of superoxide dismutase, catalase, sodium dithionite, ascorbate, dithiothreitol, EDTA, or deoxygenation with argon bubbling had no effect on the photostability of R-phycoerythrin.