Impact of oral astringent stimuli on surface charge and morphology of the protein-rich pellicle at the tooth-saliva interphase.
Impact of oral astringent stimuli on surface charge and morphology of the protein-rich pellicle at the tooth-saliva interphase.
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DOI：
10.1016/j.colsurfb.2018.11.028
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发表时间：
2019-02
期刊：
影响因子：
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通讯作者：
R. Zimmermann;J. Delius;J. Friedrichs;Susanne Stehl;T. Hofmann;C. Hannig;Melanie Rehage;C. Werner;M. Hannig
中科院分区：
文献类型：
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作者：
R. Zimmermann;J. Delius;J. Friedrichs;Susanne Stehl;T. Hofmann;C. Hannig;Melanie Rehage;C. Werner;M. Hannig
在牙齿表面上与唾液接触时形成的蛋白质薄膜层对于口腔生物膜的形成以及保护牙齿免受磨损和化学诱导的侵蚀是重要的。已知包含多酚、阳离子大分子和多价金属盐的涩味食品成分与薄膜相互作用。然而，收敛剂引起的牙齿-唾液界面的物理化学性质的变化尚未完全了解。在这里，我们提供了全面的见解，界面充电，超微结构，厚度和表面粗糙度的薄膜上形成的模型基板氧化硅（SiO2），聚四氟乙烯AF和羟基磷灰石，以及牛牙釉质之前和之后的收敛剂表没食子儿茶素没食子酸酯，单宁酸，铁（III）盐，溶菌酶和壳聚糖孵育。石英晶体微天平与耗散监测表明，未处理的pellicles形成在vitroon不同的材料的粘性行为。电动力学（流动电流）的测量结果表明，阳离子收敛剂逆转的电荷的天然表膜，而多酚没有改变在生理pH条件下的电荷。此外，透射电子显微镜和原子力显微镜表明，浓度依赖性的平均膜厚度和薄膜表面粗糙度的增加，由收敛剂引起的。唾液膜的这些多方面变化可能会沿着牙齿粗糙度的增加，这是口腔涩味复杂感觉的一部分。
The proteinaceous pellicle layer, which develops upon contact with saliva on the surface of teeth, is important for the formation of oral biofilms and for the protection of teeth from abrasion and chemically induced erosion. Astringent food ingredients comprising polyphenols, cationic macromolecules, and multivalent metal salts are known to interact with the pellicle. However, astringent-induced changes in the physicochemical properties of the tooth-saliva interphase are not yet completely understood. Here we provide comprehensive insights into interfacial charging, ultrastructure, thickness, and surface roughness of the pellicles formed on the model substrates silicon oxide (SiO2), Teflon®AF, and hydroxyapatite, as well as on bovine enamel before and after incubation with the astringents epigallocatechin gallate, tannic acid, iron(III) salt, lysozyme, and chitosan. Quartz crystal microbalance with dissipation monitoring demonstrated viscous behavior of untreated pellicles formedin vitroon the different materials. Electrokinetic (streaming current) measurements revealed that cationic astringents reverse the charge of native pellicles, whereas polyphenols did not change the charge under physiological pH condition. In addition, transmission electron microscopy and atomic force microscopy showed a concentration-dependent increase in average film thickness and pellicle surface roughness as induced by astringents. These multifaceted alterations of the salivary pellicle may come along with an increase in roughness perceived on the teeth, which is part of the complex sensations of oral astringency.