The awake behaving worm: Simultaneous imaging of neuronal activity and behavior in intact animals at millimeter scale
The awake behaving worm: Simultaneous imaging of neuronal activity and behavior in intact animals at millimeter scale
复制标题
DOI：
10.1152/jn.01050.2005
复制
发表时间：
2006-03-01
影响因子：
2.5
通讯作者：
Lockery, SR
中科院分区：
文献类型：
--
作者：
Faumont, S;Lockery, SR
基因编码的神经元活动光学探针提供了同时记录完整动物的神经元活动和行为的前景。同时成像的一个中心问题是，成像神经元所需的高倍物镜的视野通常太小，无法让实验者评估动物的整体行为状态。在这里，我们提出了一种使用显微镜解决这个问题的方法，该显微镜有两个目标专注于准备：一个高倍透镜专门用于成像神经元，低倍透镜专门用于成像行为。活动和行为的图像是同时获取的，但使用不同波长的光是分开获取的。这一新方法是利用秀丽线虫感觉神经元中表达的卡梅隆钙传感器进行测试的。我们表明，同时记录神经元活动和行为在线虫中是可行的，而且，这种记录可以揭示钙水平和行为之间的微妙、瞬时的关联，而不同时记录可能会遗漏这些关联。无论何时，只要希望从一个位置或从空间中的两个不同位置以两种不同的空间分辨率同时记录，这种新方法就很可能有用。
Genetically encoded optical probes of neuronal activity offer the prospect of simultaneous recordings of neuronal activity and behavior in intact animals. A central problem in simultaneous imaging is that the field of view of the high-power objective required for imaging the neuron is often too small to allow the experimenter to assess the overall behavioral state of the animal. Here we present a method that solves this problem using a microscope with two objectives focused on the preparation: a high-power lens dedicated to imaging the neuron and low-power lens dedicated to imaging the behavior. Images of activity and behavior are acquired simultaneously but separately using different wavelengths of light. The new approach was tested using the cameleon calcium sensor expressed in Caenorhabditis elegans sensory neurons. We show that simultaneous recordings of neuronal activity and behavior are practical in C. elegans and, moreover, that such recordings can reveal subtle, transient correlations between calcium levels and behavior that may be missed in nonsimultaneous recordings. The new method is likely to be useful whenever it would be desirable to record simultaneously at two different spatial resolutions from a single location, or from two different locations in space.