Mouse Embryonic Head as a Site for Hematopoietic Stem Cell Development
Mouse Embryonic Head as a Site for Hematopoietic Stem Cell Development
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小鼠胚胎头部作为造血干细胞发育的场所
DOI：
10.1016/j.stem.2012.07.004
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发表时间：
2012-11-02
期刊：
影响因子：
23.9
通讯作者：
Liu, Bing
中科院分区：
文献类型：
--
作者：
Li, Zhuan;Lan, Yu;Liu, Bing
在小鼠胚胎中，主动脉-性腺-中肾（AGM）区域被认为是胚胎内造血干细胞（hsc）出现的唯一位置。在这里，我们报告说，与AGM区域平行，E10.5-E11.5小鼠头部在造血干细胞进入循环之前拥有真正的造血干细胞，这是由成年受体的长期、高水平、多谱系重构和自我更新能力所定义的。妊娠中期头部血液生成的存在可以通过血管内细胞簇的出现和分选内皮细胞群的造血能力来表明。此外，通过可诱导的VE-cadherin-Cre转基因进行谱系追踪证实了头部内皮的造血能力。最重要的是，一个空间受限的谱系标记系统揭示了脑血管内皮对出生后造血干细胞和多谱系造血的生理贡献。我们得出的结论是，小鼠胚胎头是一个以前未被发现的胚胎发育中HSC出现的部位。
In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage reconstitution and self-renewal capacity in adult recipients, before HSCs enter the circulation. The presence of hemogenesis in the midgestation head is indicated by the appearance of intravascular cluster cells and the blood-forming capacity of a sorted endothelial cell population. In addition, lineage tracing via an inducible VE-cadherin-Cre transgene demonstrates the hemogenic capacity of head endothelium. Most importantly, a spatially restricted lineage labeling system reveals the physiological contribution of cerebrovascular endothelium to postnatal HSCs and multilineage hematopoiesis. We conclude that the mouse embryonic head is a previously unappreciated site for HSC emergence within the developing embryo.