Development of a handy microdiffusion device using two plastic test tubes for accurately quantifying cyanide in blood
Development of a handy microdiffusion device using two plastic test tubes for accurately quantifying cyanide in blood
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开发一种使用两个塑料试管的便携式微扩散装置，用于准确定量血液中的氰化物
DOI：
10.1007/s11419-020-00536-9
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发表时间：
2020
影响因子：
2.2
通讯作者：
Hajime Miyaguchi
中科院分区：
文献类型：
--
作者：
Yuki Okada;Hajime Miyaguchi
我们开发了一种新的准确定量血液中氰化物的装置，使用两个一次性塑料管来微扩散氰化氢，取代了传统的康威装置。考察了该装置的性能，重点考察了微扩散过程的效率，即在血液中添加氰化物后分离出的氰化物的百分比(提取效率)及其重现性。氰化物是一种众所周知的有毒阴离子，用于许多工业过程，特别是电镀过程和有机合成[1，2]。由于氰化物尽管有毒性，但相对容易获得，因此故意和意外的氰化物中毒案件继续发生[3-5]。氰化物中毒在小说中经常被提及；它是一种众所周知的致命毒药，即使对非专业人士也是如此。与氰化物有关的法律案件引起了公众的极大关注。人们可能通过故意摄入或意外接触(例如，吸入聚丙烯腈织物燃烧释放的气态氰化物或摄入富含氰基糖苷的食物)接触氰化物[6、7]。与氰化物有关的犯罪和事故导致死亡的可能性意味着有必要确定身体是否接触过氰化物。因此，氰化物分析，特别是血液中氰化物的定量分析，通常是在法医实验室进行的。
We developed a new mode of device for accurately quantifying cyanide in blood using two disposable plastic tubes for microdiffusion of hydrogen cyanide instead of a traditional Conway device. The performance of the device was investigated focusing on the efficiency of the microdiffusion process, which represented the percentage of the isolated cyanide after microdiffusion of the spiked cyanide in blood (extraction efficiency), and its reproducibility. Cyanide is a well-known toxic anion that is used in many industrial processes, particularly plating processes and organic syntheses [1, 2]. Because cyanide is relatively easy to obtain despite its toxicity, deliberate and accidental cyanide poisoning cases continue to occur [3–5]. Cyanide poisoning is frequently mentioned in novels; it is a well-known lethal poison even to non-professionals. Cyanide-related legal cases attract a great deal of public attention. People can be exposed to cyanide through intentional ingestion or through accidental exposure (eg, inhalation of gaseous cyanide released through the combustion of polyacrylonitrile fabric or ingestion of food rich in cyanoglycoside)[6, 7]. The possibility of death through cyaniderelated crimes and accidents means that it is necessary to determine whether a cadaver has been exposed to cyanide. Cyanide analyses, particularly quantitative analyses of cyanide in blood, are, therefore, routinely performed in forensic laboratories.