Decreased expression of Beclin-1 and LC3 in human lung cancer
Decreased expression of Beclin-1 and LC3 in human lung cancer
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DOI：
10.1007/s11033-011-0734-1
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发表时间：
2012-01-01
影响因子：
2.8
通讯作者：
Liu, Rong-Yu
中科院分区：
文献类型：
--
作者：
Jiang, Zi-Feng;Shao, Li-Jie;Liu, Rong-Yu
自噬是对压力的一种反向反应，最近在人类癌症中进行了研究。两个重要的自噬基因Beclin-1和Lc3在几种人类癌症中被报道。然而，Beclin-1和Lc3在肺癌中的表达尚未被研究。本研究探讨Beclin-1和Lc3在人肺癌组织中的表达及其与临床病理变化的关系。本研究共纳入40例原发性肺癌患者。实时定量聚合酶链式反应检测Beclin-1和Lc3-II的mRNA表达，免疫组织化学和免疫印迹法检测其蛋白表达水平。Beclin-1和Lc3-II在肺癌组织中的表达明显低于相应的正常肺组织。在肺癌组织中，Beclin-1和Lc3-II蛋白的表达一直较低。而Beclin-1和Lc3-II在肺癌组织中的表达与患者年龄、性别、吸烟、组织学类型、淋巴结转移和肿瘤转移分期无关。肺癌组织中Beclin-1和Lc3-II的mRNA和蛋白水平均显着降低，提示自噬可能参与了肺癌的发病机制。
Autophagy, a conversed response to stress, has recently been studied in human cancers. Two important autophagic genes-Beclin-1 and LC3 are reported in several human cancers. However, the expressions of Beclin-1 and LC3 in lung cancer have not yet been investigated. In the present study, we investigated the expression of Beclin-1 and LC3, and the relationship between the expression profile and the clinical or pathological changes in human lung cancer. 40 primary lung cancer patients are involved in present study. mRNA expressions of Beclin-1 and LC3-II were detected by Real Time PCR and the protein levels were assessed by immunohistochemistry and western blot. Relative lower expressions of Beclin-1 and LC3-II mRNA were found in the lung cancer tissues compared to counterpart normal tissues. Consistently, the lower amount of Beclin-1 and LC3-II protein was found in lung cancer tissues. However, the expressions of Beclin-1 and LC3-II in lung cancer tissues were not affected by patients' age, gender, smoking, histological type, lymph node metastasis and tumor-node-metastasis (TNM) stage. Both mRNA and protein levels of Beclin-1 and LC3-II were significantly decreased in lung cancer tissues which suggested that autophagy may be involved in the pathogenesis of lung cancer.