High‐mobility Group Box 1 Is Associated with the Inflammatory Infiltration and Alveolar Bone Destruction in Rats Experimental Periapical Lesions
High‐mobility Group Box 1 Is Associated with the Inflammatory Infiltration and Alveolar Bone Destruction in Rats Experimental Periapical Lesions
复制标题
高活动性组 Box 1 与大鼠实验性根尖周病变的炎症浸润和牙槽骨破坏相关
DOI：
10.1016/j.joen.2016.11.014
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发表时间：
2017
影响因子：
4.2
通讯作者：
B. Peng
中科院分区：
文献类型：
--
作者：
Lingshuang Liu;Jing Deng;Q. Ji;B. Peng
本研究旨在观察高迁移率族蛋白1(HMGB1)及其受体Toll样受体4(TRL4)在大鼠根尖周病变形成过程中的免疫组织化学定位。方法Wistar大鼠下颌第一磨牙髓髓暴露后35天内发生根尖周病变。在牙髓暴露后0、7、14、21、28和35天随机处死动物。结果从0天到35天，根尖周骨丢失面积逐渐增加，至35天基本稳定。第7天可见少量HMGB1、TLR4阳性细胞和破骨细胞。第7天至第28天，HMGB1和TLR4蛋白表达增加，随后保持稳定。破骨细胞数量从0天到14天成倍增加，然后从14天到35天逐渐减少。免疫荧光双重染色结果显示，HMGB1、TLR4阳性细胞分布于根尖孔周围病变周围。结论HMGB1、TLR4可能与根尖周病变的发生有关。
IntroductionThis study was conducted to observe the immunohistochemical localization of high-mobility group box 1 (HMGB1) and its receptor, Toll-like receptor 4 (TRL4), in the development of periapical lesions induced in rats. The possible role of these molecules in the pathogenesis of periapical lesions was also explored.MethodsPeriapical lesions developed within 35 days after mandibular first molar pulp exposure in Wistar rats. The animals were randomly killed at 0, 7, 14, 21, 28, and 35 days after pulp exposure. The jaws that contained the first molar were obtained and prepared for histologic analysis, enzyme histochemistry, immunohistochemistry, and double immunofluorescence staining.ResultsFrom day 0 to 35, the areas of periapical bone loss increased and appeared to be stabilized on day 35. A few HMGB1-positive, TLR4-positive cells and osteoclasts could be observed on day 7. From day 7 to 28, the HMGB1 and TLR4 protein expression increased and subsequently remained stable. The number of osteoclasts multiplied from day 0 to 14 and then gradually decreased from day 14 to 35. Double immunofluorescence staining results showed HMGB1-positive, TLR4-positive cells around periapical lesions surrounding the apical foramen.ConclusionsThus, HMGB1 and TLR4 may be associated with the pathogenesis of the periapical lesions.
影响因子：
5.5
作者：
Park, JS;Gamboni-Robertson, F;Abraham, E
通讯作者：
Abraham, E
影响因子：
5.5
作者：
Park, JS;Arcaroli, J;Abraham, E
通讯作者：
Abraham, E
影响因子：
3.5
作者：
Watanabe K;Iizuka T;Adeleke A;Pham L;Shlimon AE;Yasin M;Horvath P;Unterman TG
通讯作者：
Unterman TG