CONDUCTION-VELOCITY IN PATHWAYS FROM RETINA TO SUPERIOR COLLICULUS IN CAT - CORRELATION WITH RECEPTIVE-FIELD PROPERTIES
CONDUCTION-VELOCITY IN PATHWAYS FROM RETINA TO SUPERIOR COLLICULUS IN CAT - CORRELATION WITH RECEPTIVE-FIELD PROPERTIES
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DOI：
10.1152/jn.1973.36.3.409
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发表时间：
1973-01-01
影响因子：
2.5
通讯作者：
HOFFMANN, KP
中科院分区：
文献类型：
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作者：
HOFFMANN, KP
实验用21只成年猫（2.5-4 kg）进行，初始手术用乙醚麻醉，然后在记录期间用60:40的N20: 0混合物进行人工呼吸。动物被放置在立体定位头支架中，连续静脉滴注16毫克/小时的三硫代碘（Flaxedil， May和Baker）和1毫克/小时的二氯化氧胺（Hoffmann-LaRoche）在0.9%生理盐水（6.5毫升/小时）中麻痹（详细说明见参考文献11,23）。应用新辛弗林和阿托品扩大瞳孔；常规使用直径为3mm的人工瞳孔和眼镜镜片（通过视网膜镜检查选择，使眼睛聚焦在lm切线屏幕上）。用4 M填充nacl的微移液管记录上丘（SC）、外侧膝状核（LGN）和视网膜内的单位。当它们的尖端被破坏到大约1 -2 p外径后，这些电极的阻抗在50 Hz时从4到15兆欧ω不等。当我们从SC记录时，双极刺激电极（尖端暴露1mm，尖端分离2mm）根据Horsley-Clark坐标立体定位放置在视交叉中线（OX）两侧14.5 mm前方和1mm外侧，以及视束（OT）前方8- 9mm和10- 9mm外侧，与记录部位同侧。将第三个刺激电极放置在记录部位对侧完整眼的视盘（OD）上（参见Stone和Freeman（45）的方法）。在视网膜上记录时（46），双极刺激电极被放置在被记录眼的OX、OT和SC对侧。当在LGN中记录时，在OX和同侧SC中放置刺激电极。在三个实验中，当在SC中记录单元时，对同侧视觉皮层（VC）施加电刺激。3对导线电极（l-mm裸头）插入视觉皮层2 mm深的区域，以立体定位坐标+ 7和7前后和O-6 mm为界
Experiments were performed on 21 adult cats (2.5-4 kg), which were anesthetized with diethyl ether for initial surgery, which was followed by artificial respiration with a 60: 40 mixture of N20: 0, for the duration of the recording session. The animals were placed in a stereotaxic head holder and paralyzed with a continuous intravenous infusion of 16 mg/hr gallami ne trie thiodide(Flaxedil, May and Baker) and 1 mg/hr toxiferine dichloride (Hoffmann-LaRoche) in 0.9% saline (6.5 ml/hr)(for detailed descriptions see ref 11, 23). The pupils were dilated with Neo-Synephrine and atropine; artificial pupils of 3 mm diameter and spectacle lenses (selected by retinoscopy to focus the eye on a lm tangent screen) were routinely used. Units in the superior colliculus (SC), the lateral geniculate nucleus (LGN), and the retina were recorded using 4 M NaCl-filled micropipettes. After their tips had been broken to approximately l-2 p outer diameter, the impedance of these electrodes ranged from 4 to 15 megohms at 50 Hz. While we recorded from SC, bipolar stimulating electrodes (tip exposure 1 mm, tip separation 2 mm) were placed stereotaxically according to Horsley-Clark coordinates 14.5 mm anterior and 1 mm lateral on both sides of the midline in the optic chiasm (OX), and 8-9 mm anterior and 10-9 mm lateral in the optic tract (OT), ipsilateral to the recording site. A third stimulating electrode was placed onto the optic disc (OD) in the intact eye contralateral to the recording site (cf. method of Stone and Freeman (45)). While recording in the retina (46), bipolar stimulating electrodes were placed at OX, OT, and in the SC contralateral to the recorded eye. While recording in the LGN, stimulating electrodes were placed at OX and in the ipsilateral SC.In three experiments, when units were recorded in SC, electrical stimulation was applied to the ipsilateral visual cortex (VC). Three pairs of wire electrodes (l-mm bare tips) were inserted 2 mm deep into the area of visual cortex bounded by the stereotaxic coordinates+ 7 and-7 anterior-posterior and O-6 mm