Body weight distribution and organ size in newborn swine (sus scrofa domestica) -: A study describing an animal model for asymmetrical intrauterine growth retardation
Body weight distribution and organ size in newborn swine (sus scrofa domestica) -: A study describing an animal model for asymmetrical intrauterine growth retardation
复制标题
DOI：
10.1016/s0940-2993(98)80071-7
复制
发表时间：
1998-03-01
影响因子：
--
通讯作者：
Zwiener, U
中科院分区：
文献类型：
--
作者：
Bauer, R;Walter, B;Zwiener, U
正常生长是指遗传生长潜能的表达，既不受内部或外部因素的异常限制，也不受其促进。胎儿生长受限在人类妊娠中很常见，并与围产期发病率和死亡率增加有关。由于伦理学的限制，病理学研究必然依赖于适当的动物模型。在所介绍的研究中，将提供证据证明新生仔猪中自然发生的体重分布，从n = 512头新生仔猪中获得（约12小时）在混合的德国国内品种-“德国土地-/Edelschwein”的品种队列中连续50次分娩提供了适当的采样，为进一步的病理生理学研究提供了确定胎儿生长不同程度的统计学可靠基础。平均窝重与窝产仔数呈极显著负相关（r =-0.66，p < 0.05），同窝最低窝重与窝产仔数呈显著负相关（r =-0.64，p < 0.05）。此外，对另外53头1日龄新生仔猪进行了重量分析研究，反映了测定的自然发生的出生体重分布。体重与各器官重量之间存在显著的线性相关（相关系数值在0.45和0.98之间; p < 0.05）。中枢神经系统结构的器官重量变化最小（0.68-1.33）。骨骼和心脏显示出与体重（平均值的0.38-1.77）相似的重量变化范围（平均值的0.35-1.81和0.38-2.00）。这也在回归分析中表示，因为斜率值分别为0.99和0.97。研究的激素腺、肾脏和腹部实质器官表现出最大的重量变化范围。此外，回归分析证明，体重限制比预期的相应体重更明显。这在几乎所有这些器官中由斜率值> 1指示。IGF-1的血浆浓度与体重呈负相关（r = -0.42; p < 0.05，图4）。宫内生长迟缓（IUGR）新生仔猪的IGF-1浓度平均几乎是正常体重动物的两倍（p < 0.05），并且IUGR新生仔猪的脑重量与肝重量比增加超过2.5倍（图5A，p < 0.05）。本研究提供了一日龄仔猪自然发生的体重分布的信息，这显然是表观遗传因素的结果。重力估计清楚地表明，体重变化很可能是由胎盘功能的改变引起的。严重的改变适用于不对称的生长迟缓，这通过体重< 10（th）百分位数的动物的脑与肝的比率显著增加来证明。因此，提供的证据表明，自然发生的不对称的宫内生长受限的新生仔猪可以简单地通过体重测量，以便方便的条件下，子宫内受损的新生儿的病因动机的研究。
Normal growth is the expression of the genetic potential to growth which is neither abnormally constrained nor promoted by internal or external factors. Restricted fetal growth is common in human pregnancy and is associated with increased perinatal morbidity and mortality. Because of ethical restrictions, pathogenetical studies are necessarily dependent on appropriate animal models. In the studies presented, evidence will be provided that the naturally occurring distribution of body weight in newborn piglets, obtained from n = 512 newborn piglets (about 12 hours old) in 50 consecutive deliveries in the breed cohort of the mixed German domestic breed - "Deutsches Land-/Edelschwein" gives an appropriate sampling for providing a statistically reliable basis with which to determine different degrees of fetal growth for further pathophysiological studies intended. A strong inverse correlation (r = -0.66, p < 0.05) was found between the mean weight of the litter and the number of piglets per litter, and an inverse correlation (r = -0.64, p < 0.05) was found between the lowest weight of the littermate and the number of piglets per litter. Moreover, gravimetric investigations were made into an additional 53 one-day-old newborn piglets reflecting the naturally occurring birth weight distribution determined. A marked linear correlation between body weights and various organ weights was found (values of the correlation coefficient amounted to between 0.45 and 0.98; p < 0.05). The lowest variation of organ weights was found in the CNS structures (0.68-1.33). Skeleton and heart exhibited similar ranges of weight variation (0.35-1.81 and 0.38-2.00 of the means) to body weight (0.38-1.77 of the means). This was also expressed in the regression analysis, because the slope values were 0.99 and 0.97 respectively. The hormonal glands investigated, the kidneys, and the abdominal parenchymal organs exhibited the largest ranges of weight variation. Moreover, regression analysis gives evidence that the weight restriction was more pronounced than expected concerning respective body weight. This is indicated by slope values > 1 in almost all of those organs. Plasma concentration of IGF-1 showed an inverse correlation with body weight (r = -0.42; p < 0.05, fig 4). IGF-1 concentration of intrauterine growth retarded (IUGR) newborn piglets was in the mean nearly double that of normal weight animals (p < 0.05) and the brain weight to liver weight ratio was increased more than 2.5 times in IUGR newborn (fig 5 A, p < 0.05). This investigation provides information on the naturally occurring body weight distribution of one-day-old piglets, which was obviously a result of epigenetic factors. Gravimetrical estimation showed clearly that body weight variety is most probably caused by alterations of placental functioning. Severe alterations re suited in asymmetrical growth retardation, which was proved by a significantly increased brain to liver ratio in animals with a body weight < 10(th) centile. Thus, evidence is provided that naturally occurring asymmetrical intrauterine growth restricted newborn piglets can be identified simply by body weight measurement, so that convenient conditions are given for pathogenetically motivated studies on intrauterine compromised newborns.