MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma
MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma
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MicroRNA 筛查将循环 microRNA 鉴定为骨肉瘤的潜在生物标志物
DOI：
10.3892/ol.2015.3378
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发表时间：
2015-09-01
期刊：
影响因子：
2.9
通讯作者：
Xiao, Tao
中科院分区：
文献类型：
--
作者：
Li, Hui;Zhang, Kun;Xiao, Tao
MicroRNA（miRNAs）是一类非蛋白质编码的小分子RNA，在转录后水平调控多种基因的表达，从而控制多种生物学和病理学过程。各种循环miRNA已被鉴定为多种类型癌症和疾病的潜在诊断和预后生物标志物。本研究的目的是确定潜在的miRNA生物标志物的骨肉瘤（OS）的早期诊断和复发预测。对骨肉瘤患者和健康对照的血清进行了miRNA分析。通过逆转录-定量聚合酶链反应分析20例治疗前OS患者和20例健康个体，验证所有推定的miRNA。与健康对照组相比，OS患者血清中miR-106 a-5 p、miR-16 - 5 p、miR-20 a-5 p、miR-425- 5 p、miR-451 a、miR-25- 3 p和miR-139 - 5 p的表达均下调。受试者工作特征曲线分析表明，这7种miRNA可用作诊断生物标志物，具有区分健康队列和OS患者的能力。这些结果为使用miRNA进行OS早期血液筛查提供了新的见解。
MicroRNAs (miRNAs) are a family of small non-protein coding RNAs, which regulate the expression of a wide variety of genes at the post-transcriptional level to control numerous biological and pathological processes. Various circulating miRNAs have been identified as potential diagnostic and prognostic biomarkers in multiple types of cancer and disease. The aim of the present study was to identify potential miRNA biomarkers for the early diagnosis and relapse prediction of osteosarcoma (OS). miRNA profiling was performed on serum from patients with osteosarcoma and healthy controls. All putative miRNAs were verified by reverse transcription-quantitative polymerase chain reaction analysis of 20 pre-therapeutic OS patients and 20 healthy individuals. The expression of miR-106a-5p, miR16-5p, miR-20a-5p, miR-425-5p, miR451a, miR-25-3p and miR139-5p was demonstrated to be downregulated in the serum of OS patients when compared with that of the healthy controls. Receiver-operating characteristic curve analyses indicated that these 7 miRNAs may be used as diagnostic biomarkers with the ability to discriminate between the healthy cohort and patients with OS. These results provide novel insights into the use of miRNAs in early blood screening for OS.