Single cell sorting and cloning
Single cell sorting and cloning
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DOI：
10.1016/s0022-1759(00)00225-8
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发表时间：
2000-09-21
影响因子：
2.2
通讯作者：
Tarlinton, DM
中科院分区：
文献类型：
--
作者：
Battye, FL;Light, A;Tarlinton, DM
细胞分选仪现在允许根据一系列相当多样化的标准来选择细胞和其他物体。基于这些标准对单个细胞进行分选的额外改进已经在许多研究领域得到应用。可以通过单细胞PCR（聚合酶链反应）分选单细胞用于显微镜检查、用于培养和用于遗传分析。在实践中，在设置用于单细胞分选的仪器时，存在额外的要求以确保每个检测到的事件确实是单细胞或个体，该细胞可以经由盐水液滴可靠地分选，与其同行者分离，液滴偏转的瞄准足够精确以找到靶血管，并且细胞在到达时将未受损。在该技术的各种应用报告中，受益最大的两个领域是淋巴细胞发育和造血。在前一种情况下，淋巴细胞中的基因重排分析，无论是在前和后抗原阶段的发展，已启用的单细胞分选和PCR的组合技术。有人认为，如果没有这种伙伴关系，这些实验就不可能完成。以类似的方式，已经发现单细胞分选技术是证明造血细胞之间的前体/后代关系的完美方式，并且进一步严格证明特定细胞因子对造血系统的影响。(C)2000爱思唯尔科技有限公司。保留所有权利。
Cell sorters now allow the selection of cells and other bodies according to a range of quite diverse criteria. The additional refinement that allows the sorting of individual cells based on these criteria has seen application in many fields of research. Single cells may be sorted for microscopy, for culture and for genetic analysis by way of single cell PCR (polymerase chain reaction). In practical terms, in the setting up of an instrument for single cell sorting, there are additional requirements to ensure that each detected event is indeed a single cell or body, that this cell can be reliably sorted via saline droplet, separate from its fellow travelers, that the aiming of the droplet deflection is sufficiently precise to find the target vessel and that the cell will be undamaged on arrival. Among the diverse reported applications of the technique, two fields which have benefited greatly are lymphocyte development and haemopoiesis. In the former case, the analysis of gene rearrangements in lymphocytes, both in the pre- and post-antigenic phases of development, has been enabled by the combined technologies of single cell sorting and PCR. It is argued that such experiments could not have been done without that partnership. In a similar way, the single cell sorting technique has been found to be the perfect way to demonstrate precursor/progeny relationships between haemopoietic cells and, further, to demonstrate rigorously the effects of particular cytokines on the haemopoietic system. (C) 2000 Elsevier Science B.V. All rights reserved.