4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies
4D retrospective lineage tracing using SPIM for zebrafish organogenesis studies
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DOI：
10.1002/jbio.201000087
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Sharpe, James
中科院分区：
文献类型：
--
作者：
Swoger, Jim;Muzzopappa, Mariana;Sharpe, James
一项研究展示了一种成像框架，该框架允许在斑马鱼后侧线器官发生期间确定细胞谱系。选择性平面照明显微镜和特定荧光标记的组合允许回顾性跟踪毛细胞祖细胞，因此衍生出它们在原基内的谱系。结果表明，由于SPIM具有优越的信噪比和较低的光损伤特性，因此可以提供比旋转盘共聚焦技术更高质量的图像。这允许精确的四维谱系追踪毛细胞在几十小时的原基迁移和神经肥大发育。
A study demonstrating an imaging framework that permits the determination of cell lineages during organogenesis of the posterior lateral line in zebrafish is presented. The combination of Selective Plane Illumination Microscopy and specific fluorescent markers allows retrospective tracking of hair cell progenitors, and hence the derivation of their lineages within the primodium. It is shown that, because of its superior signal-to-noise ratio and lower photo-damaged properties, SPIM can provide significantly higher-quality images than Spinning Disk Confocal technology. This allows accurate 4D lineage tracing for the hair cells over tens of hours of primordium migration and neuromast development.