EQUATIONS FOR CALCULATION OF CHROMATOGRAPHIC FIGURES OF MERIT FOR IDEAL AND SKEWED PEAKS
EQUATIONS FOR CALCULATION OF CHROMATOGRAPHIC FIGURES OF MERIT FOR IDEAL AND SKEWED PEAKS
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DOI：
10.1021/ac00255a033
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发表时间：
1983-01-01
影响因子：
7.4
通讯作者：
DORSEY, JG
中科院分区：
文献类型：
--
作者：
FOLEY, JP;DORSEY, JG
采用指数修正高斯（EMG）模型作为色谱峰的偏态模型，建立了基于保留时间、10%峰宽（W_0 A）和经验不对称因子（B/A）的经验方程，用于准确计算理想（高斯）和偏态色谱峰的色谱优值（CFOM）。这些CFO包括：理论塔板数（观察到的效率），A/A;如果消除所有不对称性，可达到的最大效率，/V^; EMG峰参数，fQ，xQ和t;第一至第四统计矩;峰偏斜和峰过量，ys和;以及两个新的CFM-相对系统效率，RSE和相对塔板损失，RPL。当1.00< B/A< 2.76时，理论塔板数和第二中心矩（方差）的方程精确度在±1.5%以内。推荐在10%峰高处测量峰宽和B/A。近年来，人们对实验色谱峰的表征很感兴趣。表I列出了色谱峰表征中所用参数的名称、符号和通用表达式。建议将这些参数统称为色谱优值（CFOMs）。这些CFOM已经通过使用直接从色谱图进行的图形测量的手工（手动）计算或通过计算机根据数据ac-calculated来估计。
By use of the exponentially modified Gaussian (EMG) as the skewed peak model, empirical equations based solely on the graphically measurable retention time, peak width at 10% peak height, W0A, and the empirical asymmetryfactor, B/A, have been developed for the accurate and precise calculation of parameters (termed chromatographic figures of merit (CFOMs)) characterizing both Ideal (Gaussian) and skewed chromatographic peaks. These CFOMs Include: the number of theoretical plates (observed efficiency), A/SYS; the maxi-mum efficiency attainable If all asymmetry Is eliminated,/V^; the EMG peak parameters, fQ,< xQ, and t; the first through fourth statistical moments; the peak skew and peak excess, ys and; and two new CFOMs—the relative system efficiency, RSE, and the relative plate loss, RPL. Equations for the number of theoretical plates and the second central mo-ment (variance) are accurate to within±1.5% for 1.00< B/A< 2.76. Width and B/A measurements at 10% peak height are recommended.In recent years there has been considerable interest in the characterization of experimental chromatographic peaks. Presented in Table I are the names, symbols, and general expressions that have evolved for theparameters used in chromatographic peak characterization. It is proposed that these parameters be referred to collectively as chromatographic figures of merit (CFOMs). These CFOMs have been estimatedeither by hand (manual) calculation using graphical measurements made directly from the chromatogram or by a computer following data ac-