On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation
On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation
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活细胞的显微镜分期揭示了分化过程中转录爆发动力学的变化
DOI：
10.1101/2021.11.26.470114
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jeziorska D
中科院分区：
文献类型：
--
作者：
Jeziorska D
Determining the mechanisms by which genes are switched on and off during development is a key aim of current biomedical research. Gene transcription has been widely observed to occur in a discontinuous fashion, with short bursts of activity interspersed with periods of inactivity. It is currently not known if or how this dynamic behaviour changes as mammalian cells differentiate. To investigate this, using an on-microscope analysis, we monitored mouse α-globin transcription in live cells throughout erythropoiesis. We find that changes in the overall levels of α-globin transcription are most closely associated with changes in the fraction of time a gene spends in the active transcriptional state. We identify differences in the patterns of transcriptional bursting throughout differentiation, with maximal transcriptional activity occurring in the mid-phase of differentiation. Early in differentiation, we observe increased fluctuation in transcriptional activity whereas at the peak of gene expression, in early erythroblasts, transcription is relatively stable. Later during differentiation as α-globin expression declines, we again observe more variability in transcription within individual cells. We propose that the observed changes in transcriptional behaviour may reflect changes in the stability of active transcriptional compartments as gene expression is regulated during differentiation.
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影响因子：
5.3
作者：
KELLER, G;KENNEDY, M;WILES, MV
通讯作者：
WILES, MV
NGseqBasic - 用于 ATAC-seq、DNaseI-seq、Cut-and-Run 和 ChIP-seq 数据映射、高分辨率可视化和质量控制的单命令 UNIX 工具
DOI：
10.1101/393413
发表时间：
2018
期刊：
bioRxiv
影响因子：
--
作者：
Jelena M. Telenius;J. Hughes
通讯作者：
J. Hughes
影响因子：
64.5
作者：
Boija A;Klein IA;Sabari BR;Dall'Agnese A;Coffey EL;Zamudio AV;Li CH;Shrinivas K;Manteiga JC;Hannett NM;Abraham BJ;Afeyan LK;Guo YE;Rimel JK;Fant CB;Schuijers J;Lee TI;Taatjes DJ;Young RA
通讯作者：
Young RA
影响因子：
9.9
作者：
Fritzsch C;Baumgärtner S;Kuban M;Steinshorn D;Reid G;Legewie S
通讯作者：
Legewie S
影响因子：
20.3
作者：
Griffiths, Rebecca E.;Kupzig, Sabine;Anstee, David J.
通讯作者：
Anstee, David J.