A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions
A technical trick for studying proteomics in parallel to transcriptomics in symbiotic root-fungus interactions
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DOI：
10.1002/pmic.200300627
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发表时间：
2004-02-01
期刊：
影响因子：
3.4
通讯作者：
Gianinazzi, S
中科院分区：
文献类型：
--
作者：
Dumas-Gaudot, E;Amiour, N;Gianinazzi, S
我们已经开发了一个协议，其中蛋白质和mRNA可以从单个根样品进行分析。通过比较从经典的蛋白质提取过程中获得的蛋白质谱，在丛枝菌根中验证了该实验设计。这是向前迈出的一步，使同时蛋白质组和转录组分析成为可能。
We have developed a protocol in which proteins and mRNA can be analyzed from single root samples. This experimental design was validated in arbuscular mycorrhiza by comparing the proteins profiles obtained with those from a classical protein extraction process. It is a step forward to make simultaneous proteome and transcriptiome profiling possible.