Glycogen phase separation drives macromolecular rearrangement and asymmetric division in E. coli.
Glycogen phase separation drives macromolecular rearrangement and asymmetric division in E. coli.
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糖原相分离驱动大肠杆菌中的大分子重排和不对称分裂。
DOI：
10.1101/2024.04.19.590186
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Jacobs-Wagner,Christine
中科院分区：
文献类型：
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作者：
Thappeta,Yashna;Cañas-Duarte,SilviaJ;Kallem,Till;Fragasso,Alessio;Xiang,Yingjie;Gray,William;Lee,Cheyenne;Cegelski,Lynette;Jacobs-Wagner,Christine
细菌在自然界和实验室中经常经历营养限制。虽然在模型细菌大肠杆菌中，指数生长期和静止生长期得到了很好的表征，但对这两个阶段之间的过渡期间单个细胞内发生的事情知之甚少。通过定量细胞成像，我们发现在过渡期，类核和细胞分裂位点的位置变得越来越不对称。这些不对称性与蛋白质、核糖体和RNA的空间重组相结合，以核为中心定位。从活细胞成像实验，补充遗传和13 C全细胞核磁共振光谱研究的结果表明，优先积累的存储聚合物糖原在旧的细胞极导致观察到的重排和不对称分裂。体外实验表明，这些表型可能是由于糖原在拥挤的环境中相分离的倾向，因为糖原缩合物在生理拥挤条件下排除荧光蛋白。糖原相关的菌株和未来的子细胞之间的细胞大小的差异表明，糖原相分离允许细胞储存大量的葡萄糖储备，而不把它们算作细胞质空间。
Bacteria often experience nutrient limitation in nature and the laboratory. While exponential and stationary growth phases are well characterized in the model bacterium Escherichia coli, little is known about what transpires inside individual cells during the transition between these two phases. Through quantitative cell imaging, we found that the position of nucleoids and cell division sites becomes increasingly asymmetric during transition phase. These asymmetries were coupled with spatial reorganization of proteins, ribosomes, and RNAs to nucleoid-centric localizations. Results from live-cell imaging experiments, complemented with genetic and 13C whole-cell nuclear magnetic resonance spectroscopy studies, show that preferential accumulation of the storage polymer glycogen at the old cell pole leads to the observed rearrangements and asymmetric divisions. In vitro experiments suggest that these phenotypes are likely due to the propensity of glycogen to phase separate in crowded environments, as glycogen condensates exclude fluorescent proteins under physiological crowding conditions. Glycogen-associated differences in cell sizes between strains and future daughter cells suggest that glycogen phase separation allows cells to store large glucose reserves without counting them as cytoplasmic space.