Functional proteomic analysis of promyelocytic leukaemia nuclear bodies in irradiation-induced MCF-7 cells
Functional proteomic analysis of promyelocytic leukaemia nuclear bodies in irradiation-induced MCF-7 cells
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辐射诱导的 MCF-7 细胞早幼粒细胞白血病核体的功能蛋白质组学分析
DOI：
10.1093/jb/mvq105
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Sun, Zhixian
中科院分区：
文献类型：
--
作者：
Liu, Jinfeng;Song, Yi;Sun, Zhixian
已证实早幼粒细胞白血病核体(PML NBS)在DNA损伤反应(DDR)中起重要作用。照射后，PML NBS动态募集或释放参与细胞周期调节、DNA修复和细胞凋亡的重要蛋白质。由于PML蛋白是PML NBS动态组装的关键分子，本研究旨在研究经Co-60照射的MCF-7细胞中与PML相互作用的蛋白。使用CoIP、一维电泳和串联质谱学的蛋白质组学方法，使我们能够在辐射后鉴定总共124个可能与PML相关的蛋白质。生物信息学分析表明，这些蛋白质大多与PML功能相关，如转录调控、细胞周期调控、细胞死亡调控和应激反应等。B23、MVP、G3BP1和DHX9四种蛋白在电离辐射(IR)治疗前后与PML有不同的共定位。本研究中鉴定的蛋白质将显著提高我们对PML NBS在DDR中的动态组织和多功能的理解。
It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynamic assembling, we aimed to characterize the PML-interacting proteins in Co-60-irradiated MCF-7 cells. A proteomic approach using CoIP, mono-dimensional electrophoresis and tandem mass spectrometry, allowed us to identify a total of 124 proteins that may associate with PML after irradiation. Bioinformatic analysis of the identified proteins showed that most of them were related to characterized PML functions, such as transcriptional regulation, cell-cycle regulation, cell-death regulation and response to stress. Four proteins, B23, MVP, G3BP1 and DHX9, were verified to co-localize with PML differentially before and after ionizing radiation (IR) treatment. The proteins identified in this study will significantly improve our understanding of the dynamic organization and multiple functions of PML NBs in DDR.