Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord
Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord
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DOI：
10.1016/j.neures.2005.03.014
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发表时间：
2005-07-01
影响因子：
2.9
通讯作者：
Ono, Y
中科院分区：
文献类型：
--
作者：
Minaki, Y;Mizuhara, E;Ono, Y
有丝分裂后神经前体在发育中的神经管的脑室区（VZ）中产生，并立即迁移到套层（ML），在那里它们分化成成熟的神经元。虽然神经元分化和迁移的调控已被广泛研究，早期有丝分裂后的前体细胞迁移到ML的行为在很大程度上是未知的。在这项研究中，我们已经确定了Neph3作为一个特定的标志物，早期有丝分裂后的神经前体在VZ的发展脊髓。通过免疫荧光和免疫电镜对Neph3定位的分析表明，VZ中的早期神经前体不仅具有软膜连接的过程，而且还具有到达脑室的过程。这种顶端延伸的过程被证实，通过分析另一个早期的有丝分裂后标记，Dll1 mRNA，这是积极向心室运输，并积累在终端的过程。此外，adherens连接（AJs）周围的顶端形成的过程从Neph3和Dll1 mRNA阳性有丝分裂后的前体延伸。两者合计，这些观察结果表明，迁移早期有丝分裂后的神经前体在VZ的发展脊髓形成一个神经上皮细胞样双极形态，并通过AJs与相邻的细胞沟通。(c)2005年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Postmitotic neural precursors are generated in the ventricular zone (VZ) of the developing neural tube and immediately migrate to the mantle layer (ML) where they differentiate into mature neurons. Although the regulation of neuronal differentiation and migration has extensively been studied, the behavior of the early postmitotic precursors migrating toward the ML is largely unknown. In this study, we have identified Neph3 as a specific marker for early postmitotic neural precursors in the VZ of the developing spinal cord. Analysis of Neph3 localization by immunofluorescence and immunoelectron microscopy revealed that early neural precursors in the VZ possessed not only pia-connected processes but also ones that reached the ventricle. This apical extension of processes was confirmed by analyzing another early postmitotic marker, Dll1 mRNA, which was actively transported toward the ventricle and accumulated at the termini of the processes. Furthermore, adherens junctions (AJs) were formed around the apical end of processes extending from Neph3- and Dll1 mRNA-positive postmitotic precursors. Taken together, these observations suggest that migrating early postmitotic neural precursors in the VZ of the developing spinal cord form a neuroepithelial cell-like bipolar morphology and communicate with their neighboring cells through AJs. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.