Arabidopsis F-box gene FOA1 involved in ABA signaling
Arabidopsis F-box gene FOA1 involved in ABA signaling
复制标题
拟南芥F-box基因FOA1参与ABA信号传导
DOI：
10.1007/s11427-012-4332-9
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发表时间：
2012-06-01
影响因子：
9.1
通讯作者：
Liu XuanMing
中科院分区：
文献类型：
--
作者：
Peng Juan;Yu DaShi;Liu XuanMing
分析了拟南芥不同器官中 FOA1（F-box 过表达/抑制 ABA 信号传导）的表达以及对 ABA 和 NaCl 的反应。 FOA1在根中表达量较高，在茎中表达量较低，并且受ABA和NaCl快速诱导。分析了 T-DNA 插入突变马驹和 FOA1 过表达系 FOA1ox1 和 FOA1ox2 的表型。与野生型相比，马驹突变体表现出较低的发芽率、较短的根长、更多的气孔开口、增加的脯氨酸积累和对ABA的过敏。相反，过表达株系对 ABA 的敏感性低于野生型。一些 ABA 和应激反应转录因子和基因的表达水平在马驹突变体中因 ABA 的反应而发生改变。与野生型相比，突变马驹中ABA响应转录因子的表达水平较高，但ABA和应激响应基因的表达水平较低。本研究表明FOA1是ABA信号传导相关基因，可能在ABA信号传导中发挥负面作用。
The expression of FOA1 (F-box overexpressed/oppressed ABA signaling) in different organs of Arabidopsis, and in response to ABA and NaCl, was analyzed. The expression level of FOA1 is higher in the root and is lower in the stem, and is induced rapidly by ABA and NaCl. The phenotypes of T-DNA insertion mutant foal and FOA1 overexpression lines FOA1ox1 and FOA1ox2 were analyzed. The foal mutant exhibited a lower germination rate, shorter root length, more stomatal opening, increased proline accumulation and hypersensitivity to ABA compared with the wild type. In contrast, the overexpression lines showed lower sensitivity to ABA than the wild type. The expression levels of several ABA and stress-responsive transcription factors and genes were altered in the foal mutant in response to ABA. Compared with the wild type, the expression levels of ABA-responsive transcription factors were higher, but ABA and stress-responsive genes were lower in foal mutant. This study demonstrates that FOA1 is an ABA signaling-related gene, and may play a negative role in ABA signaling.