Covalent immobilization of redox protein within the mesopores of transparent conducting electrodes
Covalent immobilization of redox protein within the mesopores of transparent conducting electrodes
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DOI：
10.1016/j.electacta.2013.10.136
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发表时间：
2014-01
影响因子：
6.6
通讯作者：
V. Müller;J. Rathouský;D. Fattakhova‐Rohlfing
中科院分区：
文献类型：
--
作者：
V. Müller;J. Rathouský;D. Fattakhova‐Rohlfing
将氧化还原蛋白细胞色素c固定在高电导、高透明、大比表面积的掺锑氧化锡(ATO)介孔薄膜上。接枝是通过蛋白质与电极表面的共价连接实现的。另外，由于带正电荷的细胞色素c和带负电荷的ATO表面之间存在很强的静电相互作用，细胞色素c可以有效地吸附在ATO电极表面。电化学可寻址的细胞色素c的量与比表面积成正比，对于370 nm厚的薄膜，共价连接的蛋白质和吸附的蛋白质的比表面积分别高达440pmol/cm2和600pmol/cm2。共价结合的蛋白质比吸附的蛋白质表现出更高的稳定性。将透明导电多孔电极基质与电活性蛋白质相结合，有望开发出高效的生物光电器件，并用于生物分子的原位光谱电化学研究。
Redox protein cytochrome c was immobilized at high electrochemically accessible loading on mesoporous films of antimony doped tin oxide (ATO) exhibiting high conductivity, transparency and a large surface area. The grafting was achieved by covalent attachment of the protein to the electrode surface. Alternatively, cytochrome c was effectively adsorbed on the ATO electrode surface due to strong electrostatic interaction between the positively charged cytochrome c and the negatively charged ATO surface. The amount of electrochemically addressable cytochrome c is proportional to the specific surface area, reaching up to 440 pmol/cm2and 600 pmol/cm2for covalently attached and adsorbed protein, respectively, for the 370 nm thick films. The covalently attached protein exhibits substantially higher stability towards leaching than the adsorbed one. The combination of transparent conducting porous electrode matrix with the electroactive proteins is promising for the development of efficient bio-optoelectronic devices and for in situ spectroelectrochemical studies of biomolecules.