Structural and regulatory mutations in Vibrio parahaemolyticus type III secretion systems display variable effects on virulence.
Structural and regulatory mutations in Vibrio parahaemolyticus type III secretion systems display variable effects on virulence.
复制标题
III型弧菌溶血性纤维溶血系统中的结构和调节突变对毒力显示出可变的影响。
DOI：
10.1111/1574-6968.12619
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发表时间：
2014-12
影响因子：
2.1
通讯作者：
Orth K
中科院分区：
文献类型：
--
作者：
Calder T;de Souza Santos M;Attah V;Klimko J;Fernandez J;Salomon D;Krachler AM;Orth K
革兰氏阴性菌，副溶血性弧菌，是一个主要的原因，海洋食品来源的食物中毒在世界各地。副溶血性弧菌的致病性归因于几种毒力因子，包括两种III型分泌系统（T3SS），T3SS1和T3SS2。在此，我们比较了副溶血性弧菌POR菌株的毒力，其在任一系统的T3SS针装置中具有突变，与副溶血性弧菌CAB菌株，其在任一系统的正转录调节子中具有突变。这些菌株来源于临床RIMD 2210633菌株。我们证明，每个突变影响细菌的毒力以不同的方式。POR和CAB菌株表现出相似水平的群集运动性和T3SS效应子产生和分泌，但在T3SS2主调节基因中具有突变的CAB 3和CAB 4菌株在T3SS2诱导条件下形成减少的生物膜生长。此外，虽然POR和CAB菌株的细胞毒性相似，但CAB 2（T3SS 1调节突变体）菌株的侵袭性明显高于可比较的POR 2（T3SS 1结构突变体）菌株。总之，在T3SS1或T3SS2中产生结构或调控突变会对其他毒力系统产生不同的下游效应。了解从临床分离株产生的菌株的生物学差异对于解释和理解副溶血性弧菌的致病性至关重要。
The Gram-negative bacterium, Vibrio parahaemolyticus, is a major cause of sea-food-derived food poisoning throughout the world. The pathogenicity of V. parahaemolyticus is attributed to several virulence factors, including two type III secretion systems (T3SS), T3SS1 and T3SS2. Herein, we compare the virulence of V. parahaemolyticus POR strains, which harbor a mutation in the T3SS needle apparatus of either system, to V. parahaemolyticus CAB strains, which harbor mutations in positive transcriptional regulators of either system. These strains are derived from the clinical RIMD 2210633 strain. We demonstrate that each mutation affects the virulence of the bacterium in a different manner. POR and CAB strains exhibited similar levels of swarming motility and T3SS effector production and secretion, but the CAB3 and CAB4 strains, which harbor a mutation in the T3SS2 master regulator gene, formed reduced biofilm growth under T3SS2 inducing conditions. Additionally, while the cytotoxicity of the POR and CAB strains was similar, the CAB2 (T3SS1 regulatory mutant) strain was strikingly more invasive than the comparable POR2 (T3SS1 structural mutant) strain. In summary, creating structural or regulatory mutations in either T3SS1 or T3SS2 causes differential downstream effects on other virulence systems. Understanding the biological differences of strains created from a clinical isolate is critical for interpreting and understanding the pathogenic nature of V. parahaemolyticus.