Cellular identification of water gustatory receptor neurons and their central projection pattern in Drosophila
Cellular identification of water gustatory receptor neurons and their central projection pattern in Drosophila
复制标题
DOI：
10.1073/pnas.0502376103
复制
发表时间：
2006-01-24
影响因子：
11.1
通讯作者：
Tanimura, T
中科院分区：
文献类型：
--
作者：
Inoshita, T;Tanimura, T
水知觉对昆虫很重要，因为它们的身体很小，特别容易受到水分流失的影响。在果蝇中，味觉感受器神经元位于唇瓣、跗骨和翅缘上的味觉感受器的基部。已知典型感受器中的一个味觉感受神经元对水作出反应。为了揭示果蝇水知觉的神经机制，有必要鉴定水感受神经元及其投射模式。我们使用了GAL4增强子陷阱菌株，其中GAL4在唇瓣上的每个感器中的单个味觉受体神经元中表达。我们通过表达上游激活序列转基因，shibire（ts1），破伤风毒素轻链，或白喉毒素A链来研究这些神经元的功能。从长鼻伸展反射测试和电生理记录的结果表明，GAL4表达神经元响应于水。我们在这里表明，水受体神经元项目在食管下神经节的一个特定区域，从而揭示了果蝇的水味感觉地图。
Water perception is important for insects, because they are particularly vulnerable to water loss because their body size is small. In Drosophila, gustatory receptor neurons are located at the base of the taste sensilla on the labellum, tarsi, and wing margins. One of the gustatory receptor neurons in typical sensilla is known to respond to water. To reveal the neural mechanisms of water perception in Drosophila, it is necessary to identify water receptor neurons and their projection patterns. We used a Gal4 enhancer trap strain in which GAL4 is expressed in a single gustatory receptor neuron in each sensillum on the labellum. We investigated the function of these neurons by expressing the upstream activating sequence transgenes, shibire(ts1), tetanus toxin light chain, or diphtheria toxin A chain. Results from the proboscis extension reflex test and electrophysiological recordings indicated that the GAL4-expressing neurons respond to water. We show here that the water receptor neurons project to a specific region in the subesophageal ganglion, thus revealing the water taste sensory map in Drosophila.