Betulinic acid exerts immunoregulation and anti-tumor effect on cervical carcinoma (U14) tumor-bearing mice
Betulinic acid exerts immunoregulation and anti-tumor effect on cervical carcinoma (U14) tumor-bearing mice
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DOI：
10.1691/ph.2012.1822
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发表时间：
2012-08-01
期刊：
影响因子：
1.6
通讯作者：
Li, Jian
中科院分区：
文献类型：
--
作者：
Wang, Peijun;Li, Qingwang;Li, Jian
用于癌症治疗和预防的植物化学物质是一个重要的来源。白桦酸(betulinic acid，beta)是一种羽扇豆素型五环三萜皂苷，在以往的研究中已在一些细胞系中显示出抗肿瘤活性。本文对其在U14荷瘤小鼠体内的抗肿瘤作用及其可能机制进行了研究。结果表明，100 mg/kg和200 mg/kg的β-半胱氨酸能有效抑制荷瘤小鼠体内肿瘤生长。与对照组相比，200 mg/kg剂量组能显著提高荷瘤小鼠的IL-2和TNF-α水平，提高CD4+淋巴细胞亚群的数量和CD4+/CD8+的比值。此外，β-受体拮抗剂以剂量依赖的方式诱导荷瘤小鼠细胞凋亡，抑制Bcl2和Ki-67蛋白表达，上调caspase-8蛋白表达。其抗肿瘤作用的机制可能与诱导肿瘤细胞凋亡有关。这一过程也与机体免疫反应的改善有关。
Phytochemicals used in cancer therapy and prevention are an important source. Betulinic acid (BetA), a lupine-type pentacyclic triterpenoid saponin from plants, has shown anti-tumor activity in some cell lines in previous studies. In this paper, its anti-tumor effect and the possible mechanisms were investigated in U14 tumor-bearing mice. The results showed that BetA (100 mg/kg and 200 mg/kg) effectively suppressed tumor growth in vivo. Compared with the control group, BetA significantly improved the levels of IL-2 and TNF-alpha in tumor-bearing mice and increased the number of CD4+ lymphocytes subsets, as well as the ratio of CD4+/CD8+ at a dose of 200 mg/kg. Furthermore, treatment with BetA induced cells apoptosis in dose-dependent manner in tumor bearing mice, and inhibited the expression of Bcl-2 and Ki-67 protein while upregulated the expression of caspase-8 protein. The mechanisms by which BetA exerted anti-tumor effects might involve the induction of tumor cell apoptosis. This process is also related to improvement of body's immune response.