Seven zinc-finger transcription factors are expressed sequentially during the development of anthers in petunia
Seven zinc-finger transcription factors are expressed sequentially during the development of anthers in petunia
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DOI：
10.1046/j.1365-313x.1998.00043.x
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发表时间：
1998-02-01
期刊：
影响因子：
7.2
通讯作者：
Takatsuji, H
中科院分区：
文献类型：
--
作者：
Kobayashi, A;Sakamoto, A;Takatsuji, H
花药和花粉的发育包括几个阶段。为了鉴定参与调控花药发育相关基因表达的转录因子，我们用RT-PCR方法检测了7个在花药中表达的新锌指蛋白基因。随后的全长cDNA克隆和序列分析表明，他们都编码EPF型锌指蛋白。然而，编码的蛋白质在大小、锌指数量（两个、三个和四个）以及锌指之间的间距方面彼此非常不同。北方杂交分析表明，这些基因在花药中优先表达。此外，发现这七个基因在对应于不同发育阶段的短时间内瞬时表达。在花药发育过程中，这些基因被依次激活。考虑到这些观察结果，一个可能的机制，可以解释自主进程的花药通过这些转录因子的调节级联的发展进行了讨论。
The development of anthers and pollen involves several stages. In an attempt to identify the transcription factors that are involved in the regulation of gene expression associated with the development of anthers, seven genes for new zinc-finger proteins that are expressed in anthers were detected using RT-PCR. Subsequent cloning and sequence analysis of the full-length cDNA clones revealed that they all encoded zinc-finger proteins of the EPF type. However, the encoded proteins were very different from each other in terms of size, number of zinc fingers (two, three and four) and the spacings between the fingers. Northern blot analysis revealed that the genes were expressed preferentially in anthers. Moreover, it was found that the seven genes were expressed transiently for short periods that corresponded to different developmental stages. It appeared that the genes were activated sequentially during the development of the anther. Considering these observations, a possible mechanism that could account for the autonomous progression of the development of the anther via a regulatory cascade of these transcription factors is discussed.