MRI assessment of ischemic liver after intraportal islet transplantation.
MRI assessment of ischemic liver after intraportal islet transplantation.
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DOI：
10.1097/tp.0b013e318199c7d2
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发表时间：
2009-03-27
期刊：
影响因子：
6.2
通讯作者：
Hathout E
中科院分区：
文献类型：
--
作者：
Sakata N;Hayes P;Tan A;Chan NK;Mace J;Peverini R;Sowers L;Pearce WJ;Chinnock R;Obenaus A;Hathout E
最近有一个焦点是移植胰岛对门静脉的栓塞是早期移植物丢失的一个主要原因。由此产生的缺血会导致肝脏内细胞的坏死和/或凋亡。因此，对接受胰岛移植的肝脏进行非侵入性评估对于评估胰岛移植物的状态很重要。在这项研究中，我们利用无创性磁共振成像(MRI)来评估移植后的肝脏缺血。利用链脲佐菌素诱导的糖尿病小鼠的同基因胰岛。分别于移植后0、2、28天进行MRI和肝脏形态评估。在相似的时间点进行胰岛素、缺氧诱导因子1-α和细胞凋亡的组织学评价。移植后2天MRI发现肝脏缺血坏死区，28天未发现，并经组织学证实。在T2加权图像上，从高信号区量化肝脏损伤。胰岛素释放在移植后第2天达到高峰。门脉内胰岛移植引起的肝脏缺血的发生和逆转可通过T2加权MRI检测到。这些变化与胰岛素最大释放时期相吻合，这可能是由于部分胰岛破坏所致。我们认为，MRI作为移植物相关缺血的无创性监测，在临床上可能有助于评估门脉内胰岛移植后的肝和胰岛植入。
There is a recent focus on embolization of the portal vein by transplanted islets as a major cause of early graft loss. The resultant ischemia causes necrosis and/or apoptosis of cells within the liver. Thus, non-invasive assessment of the liver receiving the islet transplant is important to evaluate the status islet grafts. In this study, we utilized non-invasive magnetic resonance imaging (MRI) for assessment of the post-transplant ischemic liver. Syngeneic islets in streprozotocin-induced diabetic mice were utilized. MRI and morphological liver assessments were performed at 0, 2, and 28 days after transplantation. Histological assessment of insulin, hypoxia induced factor 1-α and apoptosis were undertaken at similar time-points. Ischemic/necrotic regions in the liver were detected with MRI at 2 but not at 28 days after transplantation and were confirmed histologically. Liver injury was quantified from high intensity areas on T2-weighted images. Insulin release showed a peak 2 days after transplantation. Onset and reversal of liver ischemia due to intraportal islet transplantation are detectable using T2-weighted MRI. These changes coincide with periods of maximum insulin release likely due to partial islet destruction. We propose that MRI, as a noninvasive monitor of graft-related ischemia, may be useful in assessment of liver and islet engraftment after intraportal islet transplantation in a clinical setting.