Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line?AT7-4?
Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line?AT7-4?
复制标题
DOI：
10.1016/j.hpj.2021.11.009
复制
发表时间：
2023-02-01
影响因子：
5.7
通讯作者：
Zhao，Jianjun
中科院分区：
文献类型：
--
作者：
Li，Hui;Gu，Aixia;Zhao，Jianjun
开花时间是大白菜的重要农艺性状，晚花是大白菜育种的主要目标。在前期工作中，我们获得了含有多个甘蓝有益基因的大白菜-甘蓝易位系。甘蓝特异性分子标记表明，这些基因来自甘蓝C 01染色体。本研究对一对易位系的开花时间遗传进行了研究，并分析了分子标记在后代中的传递率。通过4周春化处理，获得了开花时间比85-1晚7天左右的晚花大白菜-结球甘蓝易位系'AT 7 -4'。在前人对大白菜和结球甘蓝基因组研究的基础上，利用易位系“AT 7 -4”与大白菜“14- 36”杂交产生的自交F1群体，在F2作图群体中定位了与A01染色体顶端紧密连锁的结球甘蓝特异性分子标记。通过功能注释，在外源基因片段中发现了5个开花相关基因，并对其进行了分子标记。本研究为今后利用A-C易位系改良大白菜品种奠定了基础。
Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective. In our previous work, we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes. Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage. In this study, we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring. Consequently, we obtained the late flowering Chinese cabbage-cabbage translocation line ‘AT7–4’ in which the flowering time was later than that of ‘85–1’ by about 7 days under 4-week vernalization. Based on previous studies of the genomes of Chinese cabbage and cabbage, we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line ‘AT7–4’ and Chinese cabbage ‘14–36’. Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed. This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.