Room temperature photooxidation of β-carotene and peripheral chlorophyll in photosystem II reaction centre
Room temperature photooxidation of β-carotene and peripheral chlorophyll in photosystem II reaction centre
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光系统II反应中心β-胡萝卜素和外周叶绿素的室温光氧化
DOI：
10.1007/s11120-008-9339-5
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发表时间：
2008
影响因子：
3.7
通讯作者：
F. Vácha
中科院分区：
文献类型：
--
作者：
R. Litvín;D. Bína;F. Vácha
微分动力学吸收光谱测定光系统II反应中心和核心络合物的存在下，电子受体硅钼酸盐和铁氰化物的光化照明。含有铁氰化物的样品的光谱与含有铁氰化物和硅钼酸盐的样品的光谱不同。近红外光谱显示，在室温光化照射期间，β-胡萝卜素和外周叶绿素暂时氧化。即使在反应中心和光系统II核心复合物中β-胡萝卜素氧化活性衰减和β-胡萝卜素含量显著降低之后，外围叶绿素也被光氧化。此外，当硅钼酸盐存在时，在反应中心中的光化照射约1秒后观察到新的类胡萝卜素阳离子。在PSII核心复合物中观察到类似的结果。光照反应中心的HPLC分析揭示了几种新的类胡萝卜素，而没有新的类胡萝卜素物种在HPLC中观察到的照明核心复合物。我们的数据支持的建议，内天线的色素是一个汇的阳离子起源于光系统II反应中心。
Differential kinetic absorption spectra were measured during actinic illumination of photosystem II reaction centres and core complexes in the presence of electron acceptors silicomolybdate and ferricyanide. The spectra of samples with ferricyanide differ from those with both ferricyanide and silicomolybdate. Near-infrared spectra show temporary β-carotene and peripheral chlorophyll oxidation during room temperature actinic illumination. Peripheral chlorophyll is photooxidized even after decay of β-carotene oxidation activity and significant reduction of β-carotene content in both reaction centres and photosystem II core complexes. Besides, new carotenoid cation is observed after about 1 s of actinic illumination in the reaction centres when silicomolybdate is present. Similar result was observed in PSII core complexes. HPLC analyses of illuminated reaction centres reveal several novel carotenoids, whereas no new carotenoid species were observed in HPLC of illuminated core complexes. Our data support the proposal that pigments of inner antenna are a sink of cations originating in the photosystem II reaction centre.
光系统 II 中类胡萝卜素和叶绿素光氧化的表征。
影响因子：
2.9
作者：
Tracewell,CA;Cua,A;Stewart,DH;Bocian,DF;Brudvig,GW
通讯作者：
Brudvig,GW