Local nano-injection of fluorescent nano-beads inside C. elegans based on nanomanipulation
Local nano-injection of fluorescent nano-beads inside C. elegans based on nanomanipulation
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DOI：
10.1109/iros.2012.6386249
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发表时间：
2012-12
期刊：
影响因子：
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通讯作者：
M. Nakajima;T. Hirano;M. Kojima;N. Hisamoto;Naoya Nakanishi;H. Tajima;M. Homma;T. Fukuda
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文献类型：
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作者：
M. Nakajima;T. Hirano;M. Kojima;N. Hisamoto;Naoya Nakanishi;H. Tajima;M. Homma;T. Fukuda
本文提出了一种新的选择性纳米注射方法，使用基于纳米操纵的荧光纳米珠在体内单细胞分析。在混合显微镜下构建了纳米操作系统。混合显微镜由光学显微镜（OM）和环境扫描电子显微镜（E-SEM）组成，通过包括荧光成像在内的光学成像实现生物样品分析，通过E-SEM成像实现纳米尺度操作。基于生物纳米操纵系统，提出了一种通过纳米探针插入和纳米珠嵌入的纳米注射方法。纳米探针被设计为具有四个尖端以固定单个纳米珠。它是由聚焦离子束（FIB）工艺。它的纳米级尺寸被认为是重要的，以最小限度地损害生物标本。本研究以秀丽隐杆线虫（Caushabditis elegans，C. elegans）。它是各种疾病分析的重要模式生物之一。纳米注射技术是将荧光物质或特定生物体导入特定细胞内，用于C.优美的该系统被认为是重要的未来纳米手术系统的生命创新使用模式生物。
This paper presents a novel selective nano-injection method using fluorescent nanobeads based on nanomanipulation for in-vivo single cell analysis. The nanomanipulation system was constructed under hybrid microscope. The hybrid microscope consists of the optical microscope (OM) and environmental-scanning electron microscope (E-SEM) to realize biological specimen analysis by optical imaging including fluorescent imaging, and nano-scale manipulation by E-SEM imaging. Based on the bio-nano manipulation system, we propose a nanoinjection method by nanoprobe insertion and nanobead embeddedness. The nanoprobe was designed to have four tips to fix single nanobead. It is fabricated by focused ion beam (FIB) process. Its nano-scale size is considered to be important for minimal damage to a biological specimen. In this work, as biological specimen, the Caenorhabditis elegans (C. elegans) was used. It is one of the important model organisms for various diseases analysis. The nanoinjection technique is applied to transport fluorescent materials or specific biological organism into a specific cell for in-vivo experiment of C. elegans. The proposed system is considered to be important as future nano-surgery system for life innovation using model organism.