Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells
Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells
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DOI：
10.1590/s0100-879x2010000900003
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发表时间：
2010-09-01
影响因子：
2.3
通讯作者：
Xu, M.
中科院分区：
文献类型：
--
作者：
Ji, C.;Ren, F.;Xu, M.
鼻咽癌是中国南方常见的恶性肿瘤，病因不明。二烯丙基三硫化物(DATS)是大蒜的主要成分之一，具有很强的杀菌和杀菌作用。本研究采用四甲基偶氮唑盐[3-(4，5-dimethylthiazol-2-yl)-2，5-diphenyltetrazolium比色法、流式细胞术和Western blotting方法，研究了p38丝裂原活化蛋白激酶和半胱氨酸天冬氨酸氨基转移酶-8在DATS诱导人CNE2细胞凋亡中的作用。DATS(50、100、150 mU M)作用于CNE2细胞24 h后，细胞存活率分别为75.9%、63.4%和39.6%，凋亡率分别为24.5%、36.9%和62.4%。结果表明，DATS以剂量依赖方式诱导CNE2细胞死亡。用100mU的DATS和10mU的SB203580和Z-LETD-FMK(分别作用于p38MAPK和caspase-8)处理人CNE2细胞后，检测细胞存活率、细胞凋亡率以及p38MAPK和caspase-8活性的变化。细胞存活率分别为66.5%和68.1%，较单独用药组分别下降9.9%和11.5%。细胞凋亡率分别为31.53%和29.98%，较单独用药组分别增加9.1%和10%。结果表明，DATS可激活p38MAPK和caspase-8，但两种抑制剂对p38MAPK和caspase-8活性均有影响。综上所述，我们的研究结果表明，p38MAPK和caspase-8参与了DATS诱导人CNE2细胞凋亡的过程，并相互作用。
Nasopharyngeal carcinoma is a common malignancy in Southern China of uncertain etiologic origin. Diallyl trisulfide (DATS), one of the major components of garlic (Allium sativum), is highly bactericidal and fungicidal. In this study, we investigated the function of p38 mitogen-activated protein kinase (MAPK) and caspase-8 in DATS-induced apoptosis of human CNE2 cells using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], flow cytometry assay, and Western blotting. After CNE2 cells were treated with DATS (50, 100, or 150 mu M) for 24 h, cell viability rates were 75.9, 63.4 and 39.6%, and apoptosis rates were 24.5, 36.9, and 62.4%, respectively. The data showed that DATS induced CNE2 cell death in a dose-dependent manner. After human CNE2 cells were treated with 100 mu M DATS and inhibitors (10 mu M SB203580 and Z-LETD-FMK for p38MAPK and caspase-8, respectively), changes in cell viability and apoptosis and in p38MAPK and caspase-8 activity were detected. Cell viability rates were 66.5 and 68.1% and decreased 9.9 and 11.5% compared with inhibitor treatment alone. Apoptosis rates were 31.53 and 29.98% and increased 9.1 and 10% compared with inhibitor treatment alone. The results indicated that DATS activates p38MAPK and caspase-8, but both inhibitors have an effect on P38MAPK and caspase-8 activity. In conclusion, our data indicate that p38MAPK and caspase-8 are involved in the process of DATS-induced apoptosis in human CNE2 cells and interact with each other.