Gonadotropin-stimulated regulation of blood-follicle barrier is mediated by nitric oxide.
Gonadotropin-stimulated regulation of blood-follicle barrier is mediated by nitric oxide.
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促性腺激素刺激的血卵泡屏障调节是由一氧化氮介导的。
DOI：
10.1152/ajpendo.1995.269.2.e290
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Larsen,WJ
中科院分区：
文献类型：
--
作者：
Powers,RW;Chen,L;Russell,PT;Larsen,WJ
尽管 30 多年前首次被描述，但血滤泡屏障仍然是一个生物学之谜。在这项研究中，我们描述了血卵泡屏障在排卵刺激后对卵泡内α-胰蛋白酶抑制剂蛋白（IαI）流入的调节。我们发现 I α I 位于卵巢脉管系统内，但在给予排卵刺激之前被排除在卵泡室之外。在排卵剂量的人绒毛膜促性腺激素后几分钟内，I α I 定位在响应卵泡的卵泡液中，其中该蛋白质与新合成的富含透明质酸的卵丘细胞外基质结合并稳定。对此过程的分析表明，静脉注射硝普钠或一氧化氮合酶的过量底物 L-精氨酸，可模拟促性腺激素对 I α I 流入排卵前卵泡的卵泡室的影响。此外，静脉注射特定的一氧化氮合酶抑制剂NG-硝基-L-精氨酸甲酯和NG-硝基-L-精氨酸，可抑制促性腺激素介导的IαI的卵泡内流入，并抑制小鼠的排卵。
Although initially described over 30 years ago, the blood-follicle barrier has remained a biological enigma. In this study, we characterize the blood-follicle barrier with respect to its regulation of intrafollicular inter-alpha-trypsin inhibitor protein (I alpha I) influx after an ovulatory stimulus. We have found that I alpha I is localized within the ovarian vasculature but is excluded from the follicular compartment until an ovulatory stimulus is given. Within minutes after an ovulatory dose of human chorionic gonadotropin, I alpha I is localized within the follicular fluid of responding follicles where this protein becomes associated with and stabilizes the newly synthesized hyaluronic acid-rich cumulus extracellular matrix. Analysis of this process has shown that intravenous injection of sodium nitroprusside or excess substrate for nitric oxide synthase, L-arginine, mimics the effect of gonadotropic hormones on the influx of I alpha I into the follicular compartment of preovulatory follicles. Moreover, intravenous injection of specific nitric oxide synthase inhibitors, NG-nitro-L-arginine methyl ester and NG-nitro-L-arginine, inhibits gonadotropin-mediated intrafollicular influx of I alpha I and also inhibits ovulation in the mouse.