Enantiomer separation of amino acids in immunoaffinity micro LC-MS.
Enantiomer separation of amino acids in immunoaffinity micro LC-MS.
复制标题
在免疫亲和微型 LC-MS 中分离氨基酸的对映体。
DOI：
10.1002/chir.20286
复制
发表时间：
2006
期刊：
影响因子：
2
通讯作者：
Hofstetter,Oliver
中科院分区：
文献类型：
--
作者：
Zeleke,JessicaM;Smith,GregoryB;Hofstetter,Heike;Hofstetter,Oliver
手性免疫亲和微孔柱直接与MS检测接口，柱长和温度对一些未衍生化的芳香族和脂肪族氨基酸的对映体分离的影响进行了研究，利用抗体手性固定相，该固定相是通过将单克隆抗-D-氨基酸抗体固定在硅胶上制备的。抗体对芳香族和大体积氨基酸的更强亲和力允许在0.75 × 150 mm柱中分离此类分析物，而柱长度的增加允许分离更弱结合的化合物。通过降低温度可以方便地调节手性选择剂与分析物之间的相互作用强度。首次在基于抗体的手性固定相上使用萃取离子色谱实现了氨基酸混合物的同时对映体分离。Chirality 18：544-550，2006.© 2006 Wiley利斯公司
Chiral immunoaffinity microbore columns were directly interfaced with MS detection, and the effect of column length and temperature on the enantiomer separation of a number of underivatized aromatic and aliphatic amino acids was investigated utilizing an antibody chiral stationary phase that had been prepared by immobilizing a monoclonal anti‐D‐amino acid antibody onto silica. The stronger affinity of the antibody towards aromatic and bulky amino acids allowed separation of such analytes in a 0.75 × 150 mm column, while an increase in column length enabled separation of more weakly bound compounds. The strength of interaction between chiral selector and analytes could be modulated conveniently by lowering the temperature. For the first time, simultaneous enantiomer separation of mixtures of amino acids was achieved on antibody‐based chiral stationary phases using extracted ion chromatograms. Chirality 18:544–550, 2006. © 2006 Wiley‐Liss, Inc.