Hyperosmotic medium partially restores the growth defect and the impaired production of sterigmatocystin of an Aspergillus nidulans ΔpmtC mutant in a HogA-independent manner
Hyperosmotic medium partially restores the growth defect and the impaired production of sterigmatocystin of an Aspergillus nidulans ΔpmtC mutant in a HogA-independent manner
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高渗培养基以不依赖 HogA 的方式部分恢复构巢曲霉 ΔpmtC 突变体的生长缺陷和杂色曲霉素的产生受损
DOI：
10.1093/femsle/fnab127
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发表时间：
2021
影响因子：
2.1
通讯作者：
Shimizu Kiminori
中科院分区：
文献类型：
--
作者：
Le Thi Huynh Tram;Le Thy Nhan;Yoshimi Akira;Abe Keietsu;Imanishi-Shimizu Yumi;Shimizu Kiminori
蛋白O-甘露糖基转移酶通过将甘露糖转移到底物蛋白的丝氨酰或苏氨酰残基上来催化内质网中的O-甘露糖基化。我们曾报道过一个O-甘露糖基转移酶C（ΔpmtC）缺失突变株，该突变株营养生长和杂色曲霉素（ST）产量均受到影响。在这项研究中，我们研究了渗透压条件是否有助于Δ pmtC缺失突变体的发育过程和ST的生物合成。我们发现，在NaCl，KCl或山梨醇作为渗透稳定剂的存在下，菌丝生长和ST生产部分改善。当hogA基因被删除时，Δ pmtC缺失突变体的分生孢子在渗透胁迫条件下不能恢复。ThehogA基因编码细胞对渗透压反应所需的蛋白质。然而，ST的产量和营养生长的ΔhogAΔ pmtC双缺失体恢复高渗透压在一个HogA非依赖性的方式。
The proteinO-mannosyltransferase catalyzesO-mannosylation in the endoplasmic reticulum by transferring mannose to the seryl or threonyl residues of substrate proteins. We previously reported a deletion mutant ofO-mannosyltransferase C (ΔpmtC) inAspergillus nidulanswith impaired vegetative growth and sterigmatocystin (ST) production. In this study, we investigated whether osmotic conditions contribute to the developmental processes and ST biosynthesis of the ΔpmtCdeletion mutant. We found that hyphal growth and ST production partially improved in the presence of NaCl, KCl or sorbitol as osmotic stabilizers. Conidiation of the ΔpmtCdeletion mutant was not restored under osmotic stress conditions when thehogAgene was deleted. ThehogAgene encodes a protein required for the cellular response to osmotic pressure. However, the yield of ST and the vegetative growth of the ΔhogAΔpmtCdouble deletant was restored by high osmolarity in a HogA-independent manner.