MONTE-CARLO STUDY OF THE GTP CAP IN A 5-START HELIX MODEL OF A MICROTUBULE
MONTE-CARLO STUDY OF THE GTP CAP IN A 5-START HELIX MODEL OF A MICROTUBULE
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DOI：
10.1073/pnas.82.4.1131
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
HILL, TL
中科院分区：
文献类型：
--
作者：
CHEN, YD;HILL, TL
早期的Monte Carlo研究在微管末端的GTP帽的单螺旋模型在这里扩展到一个更现实的5开始的微管末端的螺旋模型。在早期的工作中，相变发生在微管末端：末端要么被GTP覆盖并缓慢生长，要么被脱帽并迅速缩短，并且这两种状态交替（在给定的微管蛋白浓度下）处于稳态。从Monte Carlo结果推导出2相模型的宏观速率常数。宏观速率常数导致的性能是在半定量协议与Mitchison和Kirschner的相关实验。
Earlier Monte Carlo studies on a single-helix model of the GTP cap at the end of a microtubule are extended here to a more realistic 5 start helix model of the microtubule end. As in the earlier work, phase changes occur at the microtubule end: the end is either capped with GTP and growing slowly or uncapped and shortening rapidly, and these 2 regimes alternate (at a given tubulin concentration) at steady-state. Macroscopic rate constants for the 2-phase model are deduced from the Monte Carlo results. The macroscopic rate constants lead to properties that are in semiquantitative agreement with related experiments of Mitchison and Kirschner.