Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress
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DOI：
10.1016/j.euprot.2014.05.005
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发表时间：
2014-09
影响因子：
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通讯作者：
Hongyu Ma;Liru Song;Zhigang Huang;Yan Yang;Shuang Wang;Zhankui Wang;Jianhua Tong;Weihong Gu;Hao Ma;Langtao Xiao
中科院分区：
文献类型：
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作者：
Hongyu Ma;Liru Song;Zhigang Huang;Yan Yang;Shuang Wang;Zhankui Wang;Jianhua Tong;Weihong Gu;Hao Ma;Langtao Xiao
盐胁迫是限制农业产量的主要非生物胁迫之一。为了解大豆幼苗盐响应蛋白网络，采用双向聚丙烯酰胺凝胶电泳技术分析了盐胁迫下两个不同基因型（Lee 68和杰克逊）大豆幼苗根系的蛋白质。共检测并鉴定出68个差异表达蛋白。所鉴定的蛋白质参与13种代谢途径和细胞过程。盐胁迫下，与油菜素内酯和赤霉素信号相关的蛋白质含量仅在基因型Lee 68中显著增加，脱落酸含量与该基因型呈正相关，与Ca 2+信号相关的蛋白质含量在基因型Lee 68中比在基因型杰克逊中增加更明显;在盐胁迫下，基因型Lee 68清除活性氧和维持K+/Na+稳态的能力强于基因型杰克逊。由于基因型Lee 68在文献中被描述为耐盐而杰克逊敏感，我们假设基因型Lee 68中的这些主要差异可能有助于耐盐性。结合我们前期对幼苗叶片的比较蛋白质组学分析，探讨了两种基因型幼苗叶片和根中盐响应蛋白网络的异同。这一结果将有助于大豆耐盐品种的选育。
Salinity stress is one of the major abiotic stresses that limit agricultural yield. To understand salt-responsive protein networks in soybean seedling, the extracted proteins from seedling roots of two different genotypes (Lee 68 and Jackson) were analyzed under salt stress by two-dimensional polyacrylamide gel electrophoresis. Sixty-eight differentially expressed proteins were detected and identified. The identified proteins were involved in 13 metabolic pathways and cellular processes. Proteins correlated to brassinosteroid and gilbberellin signalings were significantly increased only in the genotype Lee 68 under salt stress; abscisic acid content was positively correlated with this genotype; proteins that can be correlated to Ca2+signaling were more strongly enhanced by salt stress in the seedling roots of genotype Lee 68 than in those of genotype Jackson; moreover, genotype Lee 68 had stronger capability of reactive oxygen species scavenging and cell K+/Na+homeostasis maintaining in seedling roots than genotype Jackson under salt stress. Since the genotype Lee 68 has been described in literature as being tolerant and Jackson as sensitive, we hypothesize that these major differences in the genotype Lee 68 might contribute to salt tolerance. Combined with our previous comparative proteomics analysis on seedling leaves, the similarities and differences between the salt-responsive protein networks found in the seedling leaves and roots of both the genotypes were discussed. Such a result will be helpful in breeding of salt-tolerant soybean cultivars.