Mature Adult Mice With Exercise-Preconditioning Show Better Recovery After Intracerebral Hemorrhage.
Mature Adult Mice With Exercise-Preconditioning Show Better Recovery After Intracerebral Hemorrhage.
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DOI：
10.1161/strokeaha.120.032201
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发表时间：
2021-05
期刊：
影响因子：
8.3
通讯作者：
Arai K
中科院分区：
文献类型：
--
作者：
Kinoshita K;Hamanaka G;Ohtomo R;Takase H;Chung KK;Lok J;Lo EH;Katsuki H;Arai K
体育锻炼为几种中枢神经系统疾病提供了治疗潜力，包括中风和心血管疾病。然而，运动预适应是否以及如何影响脑出血（ICH）的预后仍是未知数。在这项研究中，我们研究了预处理对脑出血病理学的影响，在成熟的成年小鼠使用跑台运动。雄性C57 BL/6 J（25周龄）小鼠进行6周的跑步机运动，随后进行ICH诱导。结果测量包括多个时间点的各种神经功能检查和ICH诱导后8天的病灶体积评估。此外，还测定了血浆可溶性因子和病变周围吞噬小胶质细胞的数量，以确定运动预处理的作用机制。6周跑台运动预处理促进ICH诱导的小鼠神经功能缺损的恢复。此外，运动预处理的小鼠表现出较小的病变体积和吞噬小胶质细胞的数量增加。此外，与未运动的ICH小鼠相比，运动预处理的ICH小鼠血浆样品中的几种可溶性因子，包括内皮抑素、IGFBP-2和IGFBP-3、MMP-9、骨桥蛋白和正五聚蛋白-3的水平增加。这些结果表明，运动预处理的小鼠可能不会遭受严重的出血性中风损伤，因此，体育锻炼的习惯可能会改善大脑健康，即使在老年人中。
Physical exercise offers therapeutic potentials for several central nervous system disorders, including stroke and cardiovascular diseases. However, it is still mostly unknown whether and how exercise preconditioning affects the prognosis of intracerebral hemorrhage (ICH). In this study, we examined the effects of preconditioning on ICH pathology in mature adult mice using treadmill exercise. Male C57BL/6J (25-week old) mice were subjected to 6 weeks of treadmill exercise followed by ICH induction. Outcome measurements included various neurological function tests at multiple time points and the assessment of lesion volume at 8 days after ICH induction. In addition, plasma soluble factors and phagocytotic microglial numbers in the peri-lesion area were also measured to determine the mechanisms underlying the effects of exercise preconditioning. The 6-week treadmill exercise preconditioning promoted recovery from ICH-induced neurological deficits in mice. In addition, mice with exercise preconditioning showed smaller lesion volumes and increased numbers of phagocytotic microglia. Furthermore, the levels of several soluble factors, including endostatin, IGFBP-2 and -3, MMP-9, osteopontin and pentraxin-3, were increased in the plasma samples from ICH mice with exercise preconditioning compared to ICH mice without exercise. These results suggest that mice with exercise preconditioning may suffer less severe injury from hemorrhagic stroke, and therefore, a habit of physical exercise may improve brain health even in aging people.