Heparin recovers AT1 receptor and its intracellular signal transduction in cultured vascular smooth muscle cells
Heparin recovers AT1 receptor and its intracellular signal transduction in cultured vascular smooth muscle cells
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DOI：
10.1016/j.febslet.2004.11.093
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发表时间：
2005-01-03
期刊：
影响因子：
3.5
通讯作者：
Umemura, S
中科院分区：
文献类型：
--
作者：
Hashimoto, T;Kihara, M;Umemura, S
尽管血管平滑肌细胞（VSMC）广泛用于心血管研究，但它们在各种培养条件下的表型变化对于评估所获得的实验结果存在问题。血管紧张素 (Ana) 1/2 型 (ATI/AT2) 受体以及收缩蛋白和结构蛋白的水平通过培养传代而降低。本研究表明，肝素可恢复第九代 VSMC 中的 Ang 受体和分化标记物，如结蛋白、SM-22 和平滑肌 a-肌动蛋白。肝素还增强 Ang II 诱导的 ERK1/2 和 p38 激活。这些结果表明肝素处理的 VSMC 作为生理条件下血管紧张素诱导的信号转导分析模型的潜在价值。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Although vascular smooth muscle cells (VSMCs) are widely used in cardiovascular research, their phenotypic change under various culture conditions is problematic to evaluate the experimental results obtained. The levels of angiotensin (Ana) type 1/2 (ATI/AT2) receptors as well as contractile and structural proteins are degraded through culture passages. The present study demonstrated that heparin recovered Ang receptors and differentiation markers, such as desmin, SM-22 and smooth muscle a-actin in VSMCs at the ninth passage. Heparin also potenciated Ang II-induced activation for ERK1/2 and p38. These results suggest a potential value of heparin-treated VSMCs as the model for analysis of Ang-inediated signal transduction under physiological condition. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.