ETS2 function is required to maintain the transformed state of human prostate cancer cells
ETS2 function is required to maintain the transformed state of human prostate cancer cells
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DOI：
10.1038/sj.onc.1202220
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发表时间：
1998-12-03
期刊：
影响因子：
8
通讯作者：
Watson, DK
中科院分区：
文献类型：
--
作者：
Sementchenko, VI;Schweinfest, CW;Watson, DK
已经评估了 ETS2 转录因子对前列腺癌细胞转化状态的贡献。 Northern 印迹分析可轻松检测到高级人前列腺细胞系 DU145 和 PC3 中的 ETS2，但低级 LNCaP 细胞中不存在 ETS2。用反义 ETS2 载体或显性失活 ETS2 突变体稳定转染 PC3 和 DU145 前列腺细胞系，显着降低了 DU145 和 PC3 细胞在软琼脂中形成大集落的能力。因此，ETS2的存在与更多转化的表型呈正相关，并且阻断ETS2功能可以减少前列腺癌细胞的转化特性。
The contribution of the ETS2 transcription factor to the transformed state in prostate cancer cells has been assessed. Northern blot analysis easily detects ETS2 in DU145 and PC3, high grade human prostate cell lines, but ETS2 is not present in lower grade LNCaP cells. Stable transfection of PC3 and DU145 prostate cell lines with an antisense ETS2 vector or with a dominant negative ETS2 mutant significantly reduced the ability of DU145 and PC3 cells to form large colonies in soft agar. Thus, the presence of ETS2 is positively correlated with a more transformed phenotype and blockage of ETS2 function can reduce transformed properties of prostate cancer cells.