KSR, A NOVEL PROTEIN-KINASE REQUIRED FOR RAS SIGNAL-TRANSDUCTION
KSR, A NOVEL PROTEIN-KINASE REQUIRED FOR RAS SIGNAL-TRANSDUCTION
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DOI：
10.1016/0092-8674(95)90204-x
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发表时间：
1995-12-15
期刊：
影响因子：
64.5
通讯作者：
RUBIN, GM
中科院分区：
文献类型：
--
作者：
THERRIEN, M;CHANG, HC;RUBIN, GM
我们已经在果蝇中鉴定了两个基因，它们的产物需要激活的RAS以正常的效率发出信号，但似乎不能通过激活的RAF来影响信号传递。一个基因编码I型香叶基香叶转移酶的β亚基，这是一种将RAS靶向质膜的前烯基化酶，另一个基因编码一种蛋白激酶，我们称之为Ras的激酶抑制因子(KSR)。根据遗传标准，我们证明了KSR在多个受体酪氨酸激酶通路中发挥作用。我们已经分离出KSR的哺乳动物同源物，与果蝇基因一起定义了一类新的激酶。我们的结果表明，KSR是RAS信号通路中作用于RAS和RAF之间的一个普遍的、进化上保守的组成部分。
We have identified and characterized two genes in Drosophila whose products are required for activated RAS to signal with normal efficiency, but do not appear to effect signaling by activated RAF, One encodes the beta subunit of type I geranylgeranyl transferase, a prenylation enzyme essential for targeting RAS to the plasma membrane, The other encodes a protein kinase that we have named kinase suppressor of ras (ksr). By genetic criteria, we show that KSR functions in multiple receptor tyrosine kinase pathways. We have isolated mammalian homologs of KSR that, together with the Drosophila gene, define a novel class of kinases. Our results suggest that KSR is a general and evolutionarily conserved component of the RAS signaling pathway that acts between RAS and RAF.