Immunolocalization of aromatase and estrogen receptors in ram Leydig cells.
Immunolocalization of aromatase and estrogen receptors in ram Leydig cells.
复制标题
公羊 Leydig 细胞中芳香酶和雌激素受体的免疫定位。
作者：
B. Schmalz;B. Bilińska
微粒体酶，细胞色素P450芳香化酶，执行雄激素芳香化为雌激素。免疫组化法检测到了该作用的部位。性未成熟和成熟公羊为睾丸供体。分离间质细胞并在24小时内培养或将整个睾丸用于冷冻切片。对培养的细胞和切片进行免疫细胞化学或细胞化学分析。在成年公羊中，间质细胞是活性芳香化酶和雌激素受体的主要场所。而在未成熟的公羊中，观察到非常弱的活性或没有活性。因此，免疫反应芳香化酶和雌激素合成的定位已被发现是年龄依赖性的。
A microsomal enzyme, the cytochrome P450 aromatase, performs aromatization of androgens to estrogens. The site of this action has been detected by immunohistochemistry. Sexually immature and mature rams were the donors of testes. Leydig cells were isolated and cultured during 24 h or whole testes were used for cryosections. Both, cultured cells and sections were analysed immunocyto- or immunohistochemically. In adult ram, Leydig cells were the main site of an active aromatase as well as estrogen receptors. While in immature ram very weak or no activity was observed. Therefore, the localizations of immunoreactive aromatase and estrogen synthesis have been found as age-dependent.